钙离子及酸度对蛇毒抗凝血因子溶液构象的影响  

Effect of Ca 2+ Ion and Acidity on the Conformation of Anticoagulation Factor From the Venom of Agkistrodon Acutus

作　　者：吴双顶[1,2] 张祖德[1,2] 余华明[1,2] 刘清亮[1,2] 丁兰[1,2] 

机构地区：[1]中国科学技术大学应用化学系 [2]长春应用化学研究所稀土化学与物理开放实验室

出　　处：《中国科学技术大学学报》1998年第1期104-108,共5页JUSTC

基　　金：中科院长春应用化学研究所稀土化学与物理开放实验室基金;国家自然科学基金

摘　　要：用荧光光谱方法研究了Ｃａ２＋离子对抗凝血因子（ＡＣＦ）溶液构象的影响，证实了Ｃａ２＋离子不仅能与ＡＣＦ可逆结合，还能维持ＡＣＦ的结构．脱去Ｃａ２＋离子后的ＡＣＦ中约有４６％的Ｔｒｐ残基相对暴露在外．在一定的酸度范围内，酸度对ＡＣＦ的结构影响不大．The studies in this paper on the interaction of ACF with Ca 2+ ion by fluorescence spectroscopy indicate that Ca 2+ ion is very important to the main geometric structure of ACF. It is discovered that ACF can bind Ca 2+ ion reversiblly. When the Ca 2+ ion is removed from ACF,about 46 per cent of Trp residues of ACF was relatively exposed to the hydrophilic region. Acidity within the range of pH4.0￣pH9.0 exerts little effect on the structure of ACF.

关 键 词：抗凝血因子 酸度 钙离子 蛇毒 溶液构象 

分 类 号：R996.3[医药卫生—毒理学] R973.2[医药卫生—药学]