缺氧缺血性脑损伤新生大鼠脑低氧诱导因子1α mRNA的表达及参麦注射液的干预作用  

Effect of Shenmai injection on expression of hypoxia-inducible factor-1α in hypoxic-ischemic brain damage： experiment with rats

作　　者：王伟[1] 王军[1] 何艳芳[1] 陈群娥[1] 朱红梅[2] 周长美[1] 张绍美[1] 

机构地区：[1]徐州医学院附属医院新生儿科,221002 [2]徐州医学院附属医院中医科,221002

出　　处：《中华医学杂志》2009年第16期1144-1147,共4页National Medical Journal of China

基　　金：江苏省卫生厅医学科技发展基金（Z200308）;徐州市科学技术项目（200310）

摘　　要：目的研究缺氧缺血性脑损伤（HIBD）新生大鼠脑组织低氧诱导因子1α（HIF-1α）mRNA的表达及参麦注射液的干预作用。方法新生7d龄SD大鼠随机分为假手术组、生理盐水组、参麦组，又各分为缺血缺氧后2、12、24h，3、7、14d6个亚组，每亚组9只。采用反转录（RT）-PCR法检测鼠脑HIF－1α mRNA；流式细胞仪膜联蛋白V／碘化丙啶双染色法检测细胞凋亡。结果（1）缺氧缺血后2h，生理盐水组和参麦组右侧海马神经元凋亡率即均高于假手术组（均P〈0．05），于24h达到峰值后开始下降[（16．80±1．44）％、（11．95±1．13）％]，14d各组差异均无统计学意义（均P〉0．05）。参麦组海马神经元凋亡率在缺血缺氧后12、24h，3和7d均明显低于生理盐水组（均P〈0．05）。（2）缺血缺氧后2h生理盐水组、参麦组新生大鼠右侧大脑中HIF—1α mRNA的表达即均高于假手术组（均P〈0．05），于24h达峰值后开始下降[（44．32±4．03）％、（35．63±3．73）％]，14d各组表达差异均无统计学意义（均P〉0．05）；参麦组大脑中HIF-1α mRNA的表达在缺血缺氧后12、24h，3和7d均明显高于生理盐水组（均P〈0．05）。结论新生大鼠HIBD时HIF-1α mRNA表达增强，参麦注射液可提高新生大鼠HIBD后的HIF—1αmRNA的表达，减少新生大鼠缺氧缺血后神经元凋亡的发生。Objective To investigate the effects of Shemnai injection containing active principles of Ginseng and ophiopogon root on the expression of hypoxia-inducible factor 1-α （HIF-1α） in brain after hypoxic-ischemic brain damage （HIBD） . Methods 108 neonatal SD rats were randomly divided into 2 equal groups： （~） Shenmai group （Group SM）, undergoing ligation of the right common carotid artery to establish HIBD models, breathing immediately a mixed gas with 8% oxygen and 92% nitrogen for 2 h to cause HI insult, and then injected intraperitoneally with Shenmai injection 10 mg/kg once a day for 7 d, and ~） normal saline group （Group NS） undergoing ligation of the right common carotid artery to establish HIBD models, breathing immediately a mixed gas with 8% oxygen and 92% nitrogen for 2 h, and then injected intraperitoneally with NS 10 mg/kg once a day for 7 d. Another 54 neonatal rats underwent sham operation but did not undergo hypoxia as control group （Group C）, 2, 12, and 24 h, and 3, 7, and 14 d after HI insult 9 rats from each group were killed with their right hippocampal tissues taken out. Flow cytometry was used to examine the apoptotic rate of the hippocampal neurons. RT-PCR was used to detect the mRNA expression of HIF-1α. Results （ 1 ） The apoptosis rate of the right hippocampal tissues began increase 2 h after HI insult, peaked 24 h after HI, then gradually decreased, and almost returned to the original levels 14 d after HI. There was no significant differences in apoptosis rates 14 d after HI among the 3 groups （ all P 〉 0. 05）. The neuron apoptosis rates 12 h, 24 h, 3 d, and 7 d after HI of Group SM were all significantly lower than those of Group NS （ e. g 24 h： （ 11.95 ± 1.13 ） % vs （ 16. 80 ± 1.44） %, all P 〈 0. 05 ）. （2） The HIF-1α mRNA expression level in right brain began to increase 2 h after HI, peaked 24 h after HI, then gradually decreases, and returned to the original level 14 d after HI in both Group SM and Group NS; the HIF-1α mRNA

关 键 词：缺氧缺血 脑 细胞凋亡 大鼠 低氧诱导因子1Α 

分 类 号：R686[医药卫生—骨科学]