促红细胞生成素对脑出血大鼠脑血肿周围Fas相关死亡域蛋白和Caspase-8表达的影响  

Experimental studies of the effect of erythropoietin on fas-associated death domain protein and caspase-8 protein in rat with intracerebral hemorrhage

作　　者：石秋艳[1] 姜进克[1] 李倩[1] 刘超[1] 孙惠芳[1] 何俊芳[1] 张国志[2] 张瑞彪[1] 

机构地区：[1]华北煤炭医学院附属医院神经内科,唐山063000 [2]华北煤炭医学院附属医院普外科,唐山063000

出　　处：《中国综合临床》2009年第5期456-458,共3页Clinical Medicine of China

基　　金：河北省卫生厅医学科学研究重点课题资助（08182）

摘　　要：目的研究大鼠脑出血不同时期Fas相关死亡域蛋白（FADD）和Caspase-8表达及促红细胞生成素（EPO）对其的影响，探讨EPO的脑保护机制。方法sD雄性大鼠126只随机分为假手术组、脑出血组、EPO干预组各42只，各组又按不同时间点分为术后3、6、12、24、48、72h和7d共7个亚组（每个亚组6只）。采用自体血脑内注射法建立脑出血动物模型，应用免疫组化方法检测脑出血后不同时间点血肿周边脑组织中FADD和Caspase．8表达情况。结果脑出血后3hFADD和Caspase-8升高[分别为（4．66±0．46）和（15．89±1．81）]，48h达到高峰[分别为（35．88±4．24）和（45．04±3．99）]，与脑出血组比较，EPO干预组3h和48h的FADD和Caspase-8表达显著减少[分别为（3．92±0．64）和（28．24±1．90）、（13．32±2．01）和（35．08±2．85）]。结论脑出血后血肿周围细胞FADD和Caspase-8表达明显增多，EPO可能通过抑制FADD和Caspase-8的表达，对脑出血后脑组织有保护作用。Objective To study the protein expressions of Fas-associated death domain protein （FADD） and caspase-8 in rats with intracerebral hemorrhage, and the effects of erythropoietin tp reveal the mechanism of neuro-protection by EPO. Methods 126 male SD rats were randomly divided into three groups： Sham-operated group, intracerebral hemorrhage group, and EPO group. Each group was divided into seven subgroups according to the different time points （3,6,12,24,48,72 h and 7 d）. The model of intracerebral hemorrage was established in rats by intracerebral injection of autogenous blood. The protein expressions of FADD and caspas-8 in rats tissue around the hemorrhagic and the normal brain tissue were detected by immunohistochemistry. Results The protein expressions of FADD and caspase-8 were increased [ （ 4. 66 ±0. 46 ） and （ 15. 89 ± 1. 81 ） ] at 3 h after intracerebral hemorrhage, and peaked at 48 h [ （35.88 ± 4.24 ） and （45.04 ±3.99） ], the expressions of FADD and caspas-8 in the region around hematoma in EPO group significantly decreased compared with model group[ （3.92 ± 0.64） and （28.24±1.90）,（13.32±2.01） and（35.08±2.82）] at 3h and 48h. Conclusion The protein expressions of FADD and caspase-8 are markedly increased after intracerebral hemorrhage. EPO can protect the neurons by significantly reducing the expressions of FADD and caspase-8.

关 键 词：促红细胞生成素 脑出血 Fas相关死亡域蛋白 CASPASE-8 

分 类 号：R285.5[医药卫生—中药学]