同多钨酸-阿米卡星相互作用的共振瑞利散射光谱及其分析应用  

Resonance Rayleigh Scattering Spectra of Interaction Between Isopoly Tungstic Acid-Amikacin and Its Analytical Application

作　　者：胡小莉[1] 安兰香[1] 刘绍璞[1] 刘忠芳[1] 宋彦琪[1] 

机构地区：[1]西南大学化学化工学院,发光与实时分析教育部重点实验室(西南大学),重庆400715

出　　处：《应用化学》2009年第5期588-592,共5页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20475045);重庆市重点实验室(西南大学)(CSTC;2006CA8006);重庆市科委自然科学基金计划(CSTC;2007BB1359)资助项目

摘　　要：在pH值为0．65～1．10的HCl-NaAc缓冲溶液中，同多钨酸（IPT）与阿米卡星（AMK）形成离子缔合物，能引起共振瑞利散射（RRS）显著增强，并产生新的RRS光谱，其最大散射峰位于340nlTI，AMK质量浓度在1．0×10^-9～8．0×10^-8g／mL范围内与散射增强程度呈线性关系。建立了测定AMK的RRS新方法，检出限（3σ）为0．4×10^-9／mL。考察了体系的RRS和吸收光谱特征，优化了测定条件，考察了常见共存物质的干扰，方法具有良好的选择性。用于人血清中AMK的测定，结果满意。讨论了离子缔合反应和RRS增强机理。In a pH 0.65~1.10 HCl-NaAc buffer solution,isopoly tungstic acid(IPT) was reacted with amikacin(AMK) to form an ion-association complex,which resulted in great enhancement of resonance Rayleigh scattering(RRS) and the appearance of a new RRS spectrum.The maximum scattering peak was located at 340 nm. The scattering intensity was proportional to the concentration of AMK in a range of 0.001×10-6~0.08×10-6 g/mL,based on which a new RRS method for the determination of AMK was established.The method exhibited a high sensitivity and the detection limit(3σ) was 0.4×10-9 g/mL.The RRS and absorption spectral characteristics and optimum reaction conditions of the system were discussed.Effects of coexisting substances were tested,and the results demonstrated that this method had a good selectivity.It was applied to the determination of AMK in human serum samples with satisfactory results.The reaction mechanism and reasons for RRS enhancement were discussed.

关 键 词：阿米卡星 同多钨酸 共振瑞利散射 测定 

分 类 号：O657.3[理学—分析化学]