纳米微孔树脂对喜树碱和喜果苷的色谱分离  

Chromatographic Separation of Camptothecine and Vincoside-Lactam by Resins with Nanometer Micropores

作　　者：王瑞芳[1,2] 欧来良[2] 史作清[2] 

机构地区：[1]集美大学生物工程学院,厦门361021 [2]南开大学生物活性教育部重点实验室分子生物学研究所,天津300071

出　　处：《应用化学》2009年第5期593-596,共4页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金资助项目(20174017);福建省青年人才资助项目(2006F3094)

摘　　要：考察了具有不同纳米微孔结构吸附树脂对喜树果提取液中喜树碱和喜果苷的层析分离效果。静态实验结果表明,对喜果苷的吸附量与树脂的比表面积有关,比表面积达1 100 m2/g凝胶型后交联树脂具有较大的吸附量。树脂R f8对喜树碱和喜果苷的吸附分离因子最大,可达2.01。动态实验结果显示,具有大孔结构的树脂ADS-5和具有微孔结构的凝胶型后交联树脂X1对喜树碱和喜果苷的层析分离效果较差,而具有大孔-微孔结构和羰基基团的大孔后交联树脂R f8对喜树碱和喜果苷具有较好的层析分离效果,可将二者完全分离。适当表面极性与合适孔结构的R f8树脂对喜树碱和喜果苷具有优良色层分离性能。The performance of adsorption and chromatographic separation of camptothecine and vineoside-lactam by polymeric resins with different nanometer pores were studied. The static adsorption results show the adsorption capacity of vincoside-lactam is related to the specific area of the resin. The adsorption capacity of gel post-crosslinked resin with a specific area of 1 100 m2/g is larger than that of other two adsorbents. The adsorption discrepancy of Rf8 for camptothecine and vincoside-lactam was the most and the separation factor reached to 2. 01. The dynamic desorption results show that macroporous post-crosslinked resin Rf8 with micropore and macropore structures and carbonyl group can separate camptothecine and vincoside-lactam effectively via chromatography, but macroporous resin ADS-5 and gel post-crosslinked resin X1 can not. Such effective chromatographic separation of camptothecine and vincoside-lactam with resin Rf8 is ascribed to the surface character and hole structure of the resin.

关 键 词：大孔型树脂 凝胶型后交联树脂 大孔型后交联树脂 喜树碱 喜果苷 层析分离 

分 类 号：O658.9[理学—分析化学]