猪氨基酸转运载体b^(0,＋)AT-2的分子克隆及其序列分析  

Molecular Cloning and Sequence Analysis of the Amino Acid Transporter b^(0,+)AT-2 of Pigs

作　　者：职爱民[1,2] 冯定远[1] 周响艳 黄志毅[1] 邹仕庚[1] 王修启[1] 左建军[1] 叶慧[1] 王小兰[1] 刘准[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642 [2]河南农业科学院河南省动物免疫重点实验室,郑州450001 [3]东莞泛亚太生物技术有限公司,东莞523808

出　　处：《畜牧兽医学报》2009年第4期595-601,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展计划项目(973)(2004CB117501)

摘　　要：本研究克隆了b0,+AT-2的全长cDNA序列。基于NCBI发布的人和鼠b0,+AT序列,应用生物学软件进行序列比对后在同源区设计引物,获取cDNA片段;再利用RACE技术克隆了其全长cDNA序列。生物信息学分析显示:猪b0,+AT-2的全长1 488 bp,包括90 bp的3′非翻译区(UTR)和126 bp的5′UTR;编码区(CDS)编码423个氨基酸残基,分别和人、鼠b0,+AT以及猪b0,+AT-1有75.1%、71.9%和86.6%的同源性。The cloning,sequencing of porcine b0,＋AT transcript variant 2 was described in this study.Based on the sequences of human and mouse b0,＋AT published on the NCBI,we identified a partial cDNA using the primers designed after bioinformatics analysis.Using rapid amplification of cDNA ends（RACE）,the full-length cDNA encoding porcine b0,＋AT-2 was sequenced.The porcine b0,＋AT-2 cDNA was 1 488 bp long,including 90 bp 3′UTR and 126 bp 5′UTR,and CDS encoded 423 amino acids.The b0,＋AT-2 protein sequence was found to be 75.1%,71.9% and 86.6% identities with human b0,＋AT,mouse b0,＋AT and swine b0,＋AT-1.

关 键 词：氨基酸转运载体 b0 +AT-2 SLC7A9 克隆 序列分析 

分 类 号：S828[农业科学—畜牧学] Q523.8[农业科学—畜牧兽医]