大赖草总DNA转化小麦叶片mRNA差异显示技术中总RNA提取和反转录活性研究  

Study on Total RNA Extraction and Anti-transcription Activity from Transgenic Wheat Leaves with Total DNA of Leymus racemosus(Lam.) Tzvel in mRNA Differential Displaying Technology

作　　者：李静[1,2] 赵民安[1] 郝秀英[3] 王晓军[1] 曹玉锦[1,2] 胡石开[1,2] 唐晓义[1,2] 

机构地区：[1]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011 [2]中国科学院研究生院,北京100049 [3]新疆农业科学院微生物应用研究所,新疆乌鲁木齐830000

出　　处：《安徽农业科学》2009年第11期4917-4919,共3页Journal of Anhui Agricultural Sciences

基　　金：新疆农业科学院院长基金项目

摘　　要：[目的]研究大赖草总DNA转化小麦叶片mRNA差异显示技术中总RNA的质量及反转录活性。[方法]在大赖草总DNA转化小麦幼苗叶片mRNA差异显示相关试验中,利用改进的TRIzol法,从幼苗叶片中提取总RNA,紫外光谱分析,1.2%琼脂糖电泳检测,并进行随机引物RT-PCR扩增。[结果]OD260 nm/OD280 nm比值为1.95～1.98;总RNA和RT-PCR电泳条带均表现出整齐、清晰。[结论]获得的总RNA质量好、纯度高,有很高的反转录活性,完全适合于进一步的分子生物学研究。[Objective] The purpose was to study the quality and anti-transcription activity of total RNA from transgenic wheat leaves in differential displaying technology.[Method] In the relevant diferential display test of mRNA from transgenic wheat with total DNA of Leymus racemosus（Lam.） Tzvel,total RNA was extracted from seedling leaves by using modified TRIzol method.And total RNA was determined by UV-spectroscopic analysis and 1.2% agarose gel electrophoresis.Then RT-PCR amplification was made by using random primers.[Result] OD260 nm/OD280 nm was from 1.95 to 1.98.The bands of total RNA and RT-PCR were clear and orderly.[Conclusion] The obtained total RNA had good quality and high purity and anti-transcription activity.It was suitable for the further molecular biological research.

关 键 词：大赖草总DNA转化小麦 差别显示 RNA 提取 反转录活性 

分 类 号：S188[农业科学—农业基础科学]