美凤蝶非滞育蛹·滞育蛹与滞育蛹羽化成虫核酸含量比较  

Comparison of Nucleic Acid Contents Among Non-diapause Pupae,Diapause Pupae and Eclosion-adult from Diapause Pupae of Papilio memnon

作　　者：易传辉[1,2] 陈晓鸣[1] 史军义[1] 周成理[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所国家林业局资源昆虫培育与利用重点实验室,云南昆明650224 [2]云南林业职业技术学院,云南昆明650224

出　　处：《安徽农业科学》2009年第11期5018-5019,共2页Journal of Anhui Agricultural Sciences

基　　金：国家林业局引进国际先进林业科学技术计划项目(2005-4-59和2008-4-68)

摘　　要：[目的]为深入了解美凤蝶滞育机理提供依据。[方法]利用比色法,测定非滞育蛹为、滞育蛹和滞育蛹羽化成虫不同发育阶段RNA和DNA含量。[结果]非滞育蛹RNA含量为4.614 0～7.946 3μg/mg,滞育蛹为4.326 0～5.885 3μg/mg,羽化成虫初期为20.7793μg/mg;非滞育蛹DNA含量为0.448 7～0.535 0μg/mg,滞育蛹为0.452 0～0.828 3μg/mg,羽化成虫初期为1.727 0μg/mg。[结论]核酸含量与变化与发育阶段有关。[Objective] The study aimed to provide basis for knowing the diapause mechanism of Papilio memnon L.deeply.[Method] Using colorimetry,the nucleic acid content of non-diapause pupae,diapause pupae and eclosion-adult from diapause pupae were examined respectively.[Result] The result showed that the RNA content of non-diapause pupae was 4.614 0-7.946 3 μg/mg,diapause pupae was 4.326 0-5.885 3 μg/mg,and eclosion-adult from diapause pupae was 20.779 3 μg/mg.The DNA content of non-diapause pupae was 0.448 7-0.535 0 μg/mg,diapause pupae were 0.452 0-0.828 3 μg/mg,and eclosion-adult from diapause pupae was 1.727 0 μg/mg.[Conclusion]The contents and transformation of nucleic acid were related to the different stage of development.

关 键 词：美凤蝶 核酸含量 滞育 蛹 成虫 

分 类 号：Q966[生物学—昆虫学]