反相高效液相色谱法测定烟草中的类胡萝卜素及其异构体  

Determination of carotenoids and isomers in tobacco leaves by RP-HPLC

作　　者：刘少民[1] 储大燕[1] 洪深求[2] 童红武[1] 方力[3] 周桂园 常宇 

机构地区：[1]中国科学技术大学,烟草与健康研究中心,合肥市230052 [2]安徽省烟草公司,合肥230022 [3]云南烟草科学研究院,昆明650106 [4]红河烟草集团有限责任公司技术中心,弥勒652300

出　　处：《中国烟草学报》2009年第2期1-9,共9页Acta Tabacaria Sinica

基　　金：安徽省烟草公司科研基金资助(NO:ahkj2007-08)

摘　　要：采用反相高效液相色谱-二极管阵列检测器(RP-HPLC-DAD)分离和测定烟草中类胡萝卜素及其异构体的组成和含量。烟草样品经过含有0.1%丁基羟基甲苯(BHT)的丙酮溶液萃取,浓缩后,经Zorbax SBC18色谱柱分离。流动相组成:A,乙腈:水(体积比为88:12);B,乙酸乙酯。梯度洗脱程序:0～25min,100%A;25～50min,B由0%线性增加为60%;50～55min,40%A+60%B;55～60min,A由40%线性增加为100%。检测波长:450nm。进样量:10μL。流速:1.0mL/min。该方法简化了样品的前处理,共分离出烟草中11种类胡萝卜素及其异构体。类胡萝卜素物质的加标回收率为87.7%～94.6%,;相对标准偏差为3.01%～4.29%。同时研究了新鲜烟叶和烘烤后烟叶中类胡萝卜素的分布和含量,结果显示:烟叶中类胡萝卜素的组成及含量与烟叶品种、部位以及调制有关。developed for the determination of carotenoids and their isomers in tobacco leaves. Carotenoids were extracted from tobacco leaves by cold acetone solution containing 0.1% butylated hydrexytoluene （BHT）, and then were concentrated and separated on a Zorbax SB Cls column. A mobile phase of acetonitrile-H20 （88： 12, v/v） （A） and ethyl acetate （100%） （B） with the following gradient elution was used： 100% A at the beginning, maintained for 25 min, decreased to 40% A in 50 rain, maintained for 5 min and returned to 100% A in 60 min. A total of 11 constituents including carotenoids and some isomers in tobacco leaves were resolved with the flow-rate at 1.0 mL/min, injection at 10 μL and detection at 445 nm. The recoveries of carotenoids in tobacco leaves were 87.7% - 94.6%, and relative standard deviations were 3.01% - 4.29%. was successfully applied for the quantification of carotenoids in green, fresh and flue-cured tobacco leaves. The This method results show that it presents many differences not only in carotenoids contents but also in carotenoids profiles between fresh and flue-curd tobacco leaves.

关 键 词：反相高效液相色谱法 类胡萝卜素:烟草 

分 类 号：TS47[农业科学—烟草工业]