免疫抑制剂FK506对大鼠移植性肝癌肿瘤生长和脾转移的影响  

Effect of Immunosuppressant FK506 on Tumor Growth and Spleen Metastasis of Rat Transplanted Hepatoma

作　　者：黄文革[1] 包维民[2] 唐映梅[3] 刘慰华[1] 郭芬芬[1] 

机构地区：[1]中山大学实验动物中心,广州510080 [2]中山大学附属第三医院肝移植中心,广州510630 [3]昆明医学院附属二院肝病中心,昆明650101

出　　处：《中国比较医学杂志》2009年第4期35-37,F0003,共4页Chinese Journal of Comparative Medicine

摘　　要：目的观察在大鼠移植性肝癌模型中不同FK506剂量下肿瘤的大小和侵润以及脾脏转移情况,用以评价FK506对肝癌细胞增殖和转移的情况。方法在Wistar大鼠肝左叶种植walker-256细胞株成瘤的癌组织块以构建转移性肝癌模型,54只大鼠随机分为FK506低剂量组(0.5 mg/kg.d),正常剂量组(1.0 mg/kg.d)和空白对照组,测量肿瘤大小,通过病理组织学观察7、14、21 d脾脏转移发生率和中位数。结果与空白组相比,第14、21天两个剂量组肝癌体积明显增大(P<0.05);同时也出现了脾转移,第14天FK506各组转移中位数均不同。根据病理组织学,各组脾转移的发生率没有明显性差异。结论用药早期,脾脏还存在免疫功能,免疫抑制剂不能完全压制抗肿瘤效应。低剂量FK506也许有利于减少癌症脾转移,仍需进一步的探讨FK506低剂量长期使用在肝癌复发和转移的作用。Objective To observe the tumor growth and invasion, and spleen metastasis in rat transplanted hepatoma model treated at different doses of FK506 （Tacrolimus） and to evaluate the effects of FK506 on hepatoma growth and metastasis. Methods The transplanted hepatoma model was created in Wistar rats by implantation of Walker-256 tumor fragment into the left hepatic lobe. Fifty-four rats were randomly divided into FK506 low dose group （0.5 mg/kg·d）, normal dose group （1 mg/kg·d） and blank control group. The volume of hepatoma was measured. The median number and incidence rate of spleen metastasis on 7,14 and 21 d were determined by histopathology. Results Compared with the control group, the volume of hapatoma of two dose groups was significantly increased on day 14 and day 21 （P 〈 0.05）. The spleen metastasis of these rats were increased at the same time, too. The median numbers of metastases were different between FK506 groups on d 14. The histopathological examination showed no significant difference in the incidence rate of spleen metastasis among the three groups. Conclusion Immunosuppressive agent FK506 can not completely suppress the anti-tumor effect in the early treatment because of the residual immunological function of spleen. Low dose of FK506 may be beneficial to reduce cancer spleen metastasis. Further studies are needed to evaluate the effect of low dose of FK506 on recurrence and metastasis of hepatoma over a long period of time.

关 键 词：肝癌 FK506 大鼠 脾转移 

分 类 号：R33[医药卫生—人体生理学]