小鼠骨髓树突状细胞体外扩增与培养  

Expanding dendritic cells derived from mouse bone-marrow in vitro

作　　者：刘俊峰 何志旭[2] 沈冬[2] 黄瑾[2] 

机构地区：[1]华北石油总医院儿科,河北任丘062552 [2]贵阳医学院干细胞研究中心,贵州贵阳550004

出　　处：《中华肿瘤防治杂志》2009年第4期256-258,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：教育部新世纪优秀人才支持计划项目(NCET-05-0820)

摘　　要：目的:建立一种简便的体外扩增小鼠树突状细胞(DCs)的方法。方法:联合应用粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-4(IL-4)诱导培养小鼠骨髓单个核细胞分化为DCs,并从形态学、表型及功能方面对其加以检测。结果:体外诱导培养8 d后获得大量成熟的DCs,DCs表面具有典型的树枝状突起,并表达髓系DCs特异性表面分子CD11c(83.19%)、CD86(89.24%)及MHC-Ⅱ(95.25%),同时具有很强的刺激同种异基因混合淋巴细胞增殖的能力。结论:小鼠骨髓细胞体外诱导培养可生成大量功能成熟的DCs,为进一步抗肿瘤疫苗的研究奠定了基础。OBJECTIVE：To establish a method of expanding dendritic cells from mouse bone marrow in vitro. METHODS： Mononuelear cells isolated from mouse bone marrow were induced into dendritic cells by being cultured in GM-CSF and IL-4, and then examined from their morphology, phenotype and function. RESULTS： A large number of morphologically typical dendritic cells were observed after the culturing for 8 days,and high expressed CD11c （83.19%）, MHC-Ⅱ （95.25%）, and CD86 （89.24%）, and their capacity to stimulate primary T cell responses in the mixed leukocyte reaction （MLR） was shown strongly. CONCLUSION： A large number of dendritic cells can be generated by culturing the mononuclear cells derived from mouse bone marrow in vitro, establishing a foundation for producing the anti-tumour vaccine in next step.

关 键 词：骨髓细胞 树突细胞 基因扩增 细胞 培养的 

分 类 号：R392[医药卫生—免疫学]