纳米羟基磷灰石/壳聚糖-明胶复合微球的制备及性能  

Preparation and performance of nanohydroxyapatite/chitosan-gelatin composite microspheres

作　　者：黄大建[1] 张文熊[1] 刘晶冰[1] 井淇[1] 

机构地区：[1]北京工业大学材料科学与工程学院,北京市100124

出　　处：《中国组织工程研究与临床康复》2009年第16期3097-3100,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(50602002)~~

摘　　要：背景:羟基磷灰石与高分子复合材料作为组织工程材料的报道很多,但多为粉体材料或块状材料,用于修复治疗时均存在一定的局限性。目的:制备纳米羟基磷灰石/壳聚糖-明胶复合缓释微球,观察其体外释药特性。设计、时间及地点:重复测量设计,于2008-01/10在北京工业大学材料科学与工程学院生物功能高分子实验室完成。材料:纳米羟基磷灰石、壳聚糖、明胶、庆大霉素。方法:利用微波辅助法,在pH=7的条件下,制备了针状羟基磷灰石。采用W/O型复乳化-交联技术制备纳米羟基磷灰石/壳聚糖-明胶载药复合微球。主要观察指标:①纳米羟基磷灰石/壳聚糖-明胶复合微球的表面形貌、粒径分布。②载药复合微球的载药量、包封率及药物累积释放率。结果:①纳米羟基磷灰石/壳聚糖-明胶载药复合微球形态均匀,其粒径主要集中在10～30μm,壳聚糖-明胶对羟基磷灰石形成了很好的包覆。②复合微球平均载药量32.97%,平均包封率49.20%,在3d内对庆大霉素的释放达到88%左右。结论:所制备的纳米羟基磷灰石/壳聚糖-明胶载药复合微球形态均匀,粒径分布窄,再分散性好,3d内能维持有效的药物浓度。BACKGROUND： Hydroxyapatite/polymer composite materials have been widely reported as tissue engineering materials; however, they are limited in repairing treatment because of the powder or piece in shape. OBJECTIVE： To prepare the nanohydroxyapatite （HAP）/chitosan-gelatin （CG） composite microspheres, and to observe the general drug release in vitro. DESIGN, TIME AND SETTING： Repeated measurement study was performed at Laboratory of Biological Functional Polymer, College of Material Science and Chemical Engineering, Beijing University of Technology between January and October 2008. MATERIALS： Nanohydroxyapatite, chitosan, gelatin, and gentamicin were used in this study. METHODS： Microwave irradiation method was used to synthesize aciform hydroxyapatite （pH=7）, and W/O combined with multiple emulsification chemical crosslink technique was used to prepare nanohydroxyapatite/chitosan-gelatin composite microspheres. MAIN OUTCOME MEASURES： （1） Surface morphology and particle diameter distribution; （2） drug loading, entrapment efficiency, and cumulative release rate. RESULTS： （1） The composite microspheres were spherical in shape, and the particle diameter ranged from 10 to 30 μm. Hydroxyapatite was greatly embedded by chitosan-gelatin. （2） The average drug loading was 32.97%, the average entrapment efficiency was 49.20%, and the cumulative release rate to gentamincin was near 88% within 3 days. CONCLUSION： The composite microspheres exhibit well-distributed morphology, narrow particle diameter distribution and good redispersibility. They can maintain an therapeutic concentration within 3 days.

关 键 词：纳米羟基磷灰石 壳聚糖 明胶 庆大霉素 骨修复 

分 类 号：R318[医药卫生—生物医学工程]