自制新型多器官保存液低温保存小型猪肾  

Cryopreservation of mini pig kidney with the self-designed new multi-organ preservation solution

作　　者：袁清[1] 赵闻雨[1] 熊海云[1] 罗明[2] 王立明[1] 朱有华[1] 

机构地区：[1]解放军第二军医大学长征医院器官移植科,解放军器官移植研究所,上海市200003 [2]上海市浦东新区公利医院泌尿外科,上海市200135

出　　处：《中国组织工程研究与临床康复》2009年第18期3467-3470,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：上海科委基金(024919006)~~

摘　　要：背景:在前期实验的基础上,此次实验成功配制了含聚乙二醇的新型上海多器官保存液。目的:以普通器官保存液为对照,验证自制含聚乙二醇的上海多器官保存液低温保存小型猪肾的效果。设计、时间及地点:随机分组,对照实验观察,2006-05/2007-07在解放军器官移植研究所移植实验室完成。材料:健康成年实验用巴马小型猪12头。自制新型新型上海多器官保存液,主要含聚乙二醇,相对分子质量20000。pH7.43±0.10,渗透压825～900kPa,UW液,购自美国Bristol-Myers Squibb公司。方法:巴马小型猪肾分别以4℃器官保存液或上海多器官保存液原位灌注后离体保存24,48,72h。保存终点测保存液pH值及乳酸脱氢酶含量,左肾取标本行组织学检查,右肾采用体外非循环猪肝/肾灌注系统灌注30,60,120min测灌注液乳酸脱氢酶含量。灌注终点取肾皮质标本测丙二醛含量和超氧化物歧化酶活性。主要观察指标:光镜、电镜观察肾冷保存后的形态学变化;灌注液及保存液乳酸脱氢酶含量;肾皮质标本测丙二醛浓度和超氧化物歧化酶活性;肾组织细胞凋亡指数。结果:保存48,72h后,器官保存液组保存液pH值高于上海多器官保存液组(P<0.05)。保存72h终点保存液及再灌注30,60,120min灌注液中乳酸脱氢酶活性器官保存液组高于上海多器官保存液组(P<0.05)。同时间点两组肾组织光镜、电镜下形态学改变、肾皮质凋亡指数、超氧化物歧化酶、丙二醛含量差异均无显著性。结论:新型上海多器官保存液对小型猪肾脏低温保存效果与普通器官保存液基本一致,对缺氧代谢器官细胞内酸中毒的缓冲能力以及对低温和缺血再灌注损伤的保护作用优于普通器官保存液。BACKGROUND： A new multi-organ preservation solution mainly containing polyethylene glycol has been successfully prepared on the basis of previous studies. OBJECTIVE： Using University of Wisconsin （UW） solution as a standard control, to verify the effect of Shanghai-multi-organ （SMO） solution, a self-designed multiple organ preservation solution mainly containing polyethylene glycol for cryopreservation of kidney of mini pig. DESIGN, TIME AND SETTING： A randomized block control observational experiment was performed at Transplantation Laboratory of Organ Transplantation Institute of Chinese PLA from May 2006 to July 2007. MATERIALS： A total of 12 healthy adult BA-Ma mini pigs were selected in the experiment. SMO solution（relative molecular mass： 20000; pH： 7.43±0.10; osmotic pressure： 825 900 kPa） mainly containing polyethylene glycol was used in the experiment. UW solution was the product of Bristol-Myers Squibb. METHODS： The kidneys of BA-Ma mini pigs were perfused in situ and preserved in vitro in SMO or UW solution at 4 ℃ for 24, 48 and 72 hours. The pH value and lactate dehydrogenase （LDH） content of the preservation solution was measured. The left kidneys were extracted for histological examination. The right kidneys were perfused by using in vitro perfusion system for 30, 60 and 120 minutes, and then the LDH content were measured. The renal cortical samples were obtained to determine the content of malondialdehyde （MDA） and activity of superoxide dismutase （SOD）. MAIN OUTCOME MEASURES： The morphologic changes were observed under light and electronic microscopes. The contents of LDH in perfusate and preservation solution, as well as the content of MDA and activity of SOD in renal cortical samples were measured. Apoptosis index of kidney cortical cells were determined. RESULTS： The pH value in the SMO solution was significantly higher than that in the UW solution at 48 and 72 hours after cryopreservation （P 〈 0.05）. The contents of LDH in preserva

关 键 词：器官保存液 肾 巴马小型猪 聚乙二醇 

分 类 号：R318[医药卫生—生物医学工程]