猪繁殖与呼吸综合征病毒流行株Jiangxi-3的全基因组序列分析  

Whole-Genome Analysis of PRRSV Isolate Jiangxi-3

作　　者：王旭荣[1,2,3] 张彩勤[1,3] 赵炳武[1,4] 张春红[1] 杨增岐[3] 宋长绪[1] 

机构地区：[1]广东省农科院兽医研究所,广州510640 [2]中国农科院兰州畜牧与兽药研究所,兰州730050 [3]西北农林科技大学动物医学院,杨凌712100 [4]内蒙古农业大学动物科学与医学学院,呼和浩特010018

出　　处：《上海交通大学学报（农业科学版）》2009年第2期101-107,共7页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家食品安全重大专项(2001BA804A31);广东省农业科技攻关(2003B21404;20052490100和2006B20301013);广东省自然科学基金(04002083);广东省自然基金重点项目(06105311)

摘　　要：通过RT-PCR分段扩增PRRSV流行株Jiangxi-3的基因组,参照VR2332序列,根据各片段之间相重叠的区域,将扩增片段的序列依次拼接获得Jiangxi-3完整的全基因组,并通过DNAStar软件对其进行分析。结果表明:Jiangxi-3的Nsp2推导氨基酸发生30个氨基酸的不连续缺失,与HB-1(sh)/2002相比,Nsp2推导的第480位氨基酸由D突变为E,缺失的位置为第481位氨基酸和532～560位氨基酸;Jiangxi-3与2006年Tian Kegong等从江西"高热病"病死猪体内分离的毒株JXA1的各个结构蛋白阅读框的氨基酸同源性为98.0%～100%;Jiangxi-3与HB-1(sh)/2002、VR2332、RespPRRSMLV的Nsp2推导的氨基酸同源性分别为94.7%、77.8%、77.7%,由遗传系统发育树可知与2006年以前分离的毒株相比,Jiangxi-3与HB-1(sh)/2002的亲缘关系较近。说明Jiangxi-3和JXA1极可能由同一毒株HB-1(sh)/2002演化而来。Whole-genome sequence of isolate Jiangxi-3 were obtained by splicing of many RT-PCR fragments according to the sequence of VR2332. Whole-genome analysis revealed that Jiangxi-3 isolate are highly homologous to HB-1（sh）/2002, a Chinese strain of PRRSV （94.7% amino acids identity of Nsp2）. More importantly, we observed a unique molecular hallmark in the viral isolate, namely a discontinuous deletion of 30 amino acids in nonstructural protein 2 （Nsp2）. Compared with HB-1（sh）/2002, 480 aa was mutated （D→E） and the position of deletion were 481 aa and 532-560 aa. Compared with JXA1 isolate 98.0%-100% homology for every gene reading frame, what is a Chinese strain of PRRSV isolated from ＂high fever＂ disease in pig herds by Veterinary Diagnostic Department China Animal Disease Control Center in 2006. Jiangxi-3 shared 94.7%, 77.8%, 77.7% amino acid homology for Nsp2 compared with HB-1 （sh）/2002, VR2332, RespPRRS MLV. The results showed that Jiangxi-3 and JXA1 were probably evolved from HB- 1 （sh）/2002.

关 键 词：猪繁殖与呼吸综合征病毒 Jiangxi-3 全基因组 序列分析 

分 类 号：S852.651[农业科学—基础兽医学]