根结线虫诱导型启动子RKNIP的克隆及序列分析  

Clone and Sequence Analysis of Root-knot Nematode Induced Promoter(RKNIP)

作　　者：霍雨猛[1] 王伟[1] 徐苑芳 何启伟[1] 金国良[3] 侯丽霞[1] 赵双宜[4] 

机构地区：[1]山东省农业科学院蔬菜研究所,山东济南250100 [2]济南北方农艺科学研究所,山东济南250100 [3]青岛农业大学,山东青岛266109 [4]山东大学生命科学学院,山东济南250100

出　　处：《山东农业科学》2009年第4期5-8,15,共5页Shandong Agricultural Sciences

基　　金：山东省农业良种工程项目

摘　　要：通过对烟草根特异性基因TobRB7调控序列的分析,构建了受根结线虫诱导的特异性表达启动子RKNIP的克隆载体,其中RKNIP500已于GenBank注册,注册号为DQ486886。模序分析表明,RKNIP500具有双向启动子的功能,而RKNIP300不具有启动子的功能,但存在大量与启动子功能相关的元件,特别是存在25个根特异性表达的元件。In this paper, the clone vector of pGEM - T - RKNIP was constructed through analyzing the regulator sequence of TobRB7. The sequence of the RKNIP500 has been applied to GenBank, and the accession number is DQ486886. Then the motifs of the RKNIP500 and RKNIP300 were analyzed. The results showed that RKNIP500 had the function of bidirectional promoter, while RKNIP300 had no function of promoter. But RKNIP300 had many elements relative to promoter, especially 25 elements of root specific expression.

关 键 词：根结线虫 诱导型启动子 RKNIP 

分 类 号：Q78[生物学—分子生物学]