DNA加合物作为氯乙烯接触生物标志物的研究  

Study on DNA adduct as the exposure biomarker to vinyl chloride monomer in workers

作　　者：缪文彬[1,2] 王威[1] 郑盈佳[1] 冀芳[1] 夏昭林[1] 

机构地区：[1]复旦大学公共卫生学院劳动卫生毒理学教研室与公共卫生安全教育部重点实验室,上海200032 [2]上海出入境检验检疫局,上海200135

出　　处：《中国工业医学杂志》2009年第2期87-90,共4页Chinese Journal of Industrial Medicine

基　　金：国家自然科学基金(30671740);国家973项目(2002CB512909)

摘　　要：目的探讨DNA加合物作为氯乙烯作业工人的暴露生物标志物的可行性。方法采用酶联免疫吸附法对43名氯乙烯接触工人淋巴细胞中1-氮-6-乙烯(脱氧)腺嘌呤含量进行测定。结果相关分析和偏相关分析结果显示,1-氮-6-乙烯(脱氧)腺嘌呤含量与累积接触剂量存在相关关系(P<0.05)。未发现年龄、性别、吸烟和饮酒对1-氮-6-乙烯(脱氧)腺嘌呤含量的影响。累积接触剂量>23 740 mg组1-氮-6-乙烯(脱氧)腺嘌呤含量显著高于<8 150mg组,趋势卡方检验结果显示1-氮-6-乙烯(脱氧)腺嘌呤的形成与累积接触剂量存在明显的剂量-反应关系。结论1-氮-6-乙烯(脱氧)腺嘌呤可作为氯乙烯暴露的接触生物标志物。Objective To explore the possibility of DNA adduet to be the exposure biomarker to vinyl chloride monomer in workers. Methods Levels of 1, N （ 6 ） -etheno （deoxy） adenosine （edA） in the peripheral leukocytes were determined by enzymelinked immunosorbent assay （ELISA） in 43 VCM-exposed workers. Results Significant correlation was found between edA and VCM exposure by correlation analysis and partial correlation analysis （ P 〈 0.05 ）. Effects of gender, age, smoking and alcohol drinking on the levels of edA were not found. Levels of edA in workers with exposure dose more than 23 740 mg were significantly higher than that less than 8 150 mg （P =0. 006）. DNA adduct showed significantly increase with VCM exposure doses as a dose-dependant manner. Conclusion εdA could be a biomarker of VCM exposure in workers.

关 键 词：氯乙烯 DNA加合物 累积接触剂量 接触生物标志物 

分 类 号：R135.1[医药卫生—劳动卫生] R99[医药卫生—公共卫生与预防医学]