人溶菌酶基因的克隆及其真核表达载体的构建  

Cloning and Construction of Eukaryotic Expression Vector of Human Lysozyme Gene

作　　者：余露露[1] 潘春留[1] 关伟军[2] 卿素珠[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨陵712100 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《动物医学进展》2009年第4期5-7,共3页Progress In Veterinary Medicine

基　　金：国家自然科技资源平台建设项目(2005DKA21101)

摘　　要：通过设计人溶菌酶(hLYZ)特异性引物,Trizol试剂盒从人体胎盘组织中提取总RNA,RT-PCR法从总RNA中扩增出目的基因hLYZ,将其与PGEM-T-easy载体连接,转化大肠埃希菌DH5α感受态细胞,经酶切法连接到真核表达载体,构建重组的质粒表达载体pEGFP-N3-hLYZ,获得了hLYZ全长cD-NA并构建出hLYZ真核表达载体。测序表明克隆的hLYZ全长cDNA与GenBank中hLYZ序列完全一致。研究结果为进一步研究制作hLYZ的抗菌作用机制奠定了基础。The hLYZ full-length eDNA was amplified through RT-PCR from total RNA in placenta tissues of human. The hLYz eDNA was insented in PGEM-T-easy expression vector, The combinant plasmid was transformed to DH5α competent cells. The plasmid expression vector pEGFP-N3-hLYZ was obtained. The results suggested that we successfully gained full-length eDNA of hLYZ and constructed its eukaryotic expression vector. The sequencing results were aligned with sequence registered in GenBank. The results laid a foundation for further study on the antibacterial mecharzism of hLYZ.

关 键 词：人溶菌酶 克隆 真核表达载体 

分 类 号：Q785[生物学—分子生物学] Q786