Dot-ELISA检测兔疥螨抗体方法的建立和应用  

Establishment and Application of Dot-ELISA for Detecting Antibodies against Sarcoptes scabiei var.cuniculi

作　　者：郝桂英[1,2] 杨光友[1] 古小彬[1] 

机构地区：[1]四川农业大学动物医学院,四川雅安625014 [2]西昌学院动物科学系,四川西昌615013

出　　处：《动物医学进展》2009年第4期8-13,共6页Progress In Veterinary Medicine

摘　　要：将从病兔痂皮内收集的疥螨经研磨、冻融、离心后,制成可溶性抗原,作为诊断抗原,建立Dot-ELISA方法检测兔疥螨血清抗体。研究确定了该方法的最佳工作条件。制备的诊断膜片特异性强,不与兔瘟病毒、兔大肠埃希菌、兔附红细胞体等阳性血清反应。膜片具有良好的灵敏性,高免血清作1∶210稀释亦能检出;重复性试验表明该法重复性良好。诊断膜片在4℃保存5个月其检测活性不变。结果表明,建立的Dot-ELISA可用于免疥螨抗体的检测。The soluble proteins of sarcoptes scabiei var. cuniculi were extracted by repeating freezing thaw, grinding and centrifugation, which was selected as diaphragm-carried antigen in Dot-ELISA. Through repeated experiments, the optimal trial conditions of Dot-ELISA were determined. The specificity was proved by the blocking and cross-reaction tests. The diaphragm did not react with the antibodies against RHDV, E. coli Eperythrozoon and fungi. The diaphragm had the ability to detect the positive serum when it was diluted to 1 ： 21^-10 and so it had good sensitivity. The repeatability test proved that the method or the diagnostic diaphragm was stable. Stored at 4 ℃ for at least 5 months, the diagnostic diaphragm＇s sensitivity and specificity didn＇t change, so it had good stability. Results have shown that Dot-ELISA can be used to defect sarcoptes scabieivar cuniculi .

关 键 词：斑点酶联免疫吸附试验 兔疥螨 抗体检测 

分 类 号：S852.746[农业科学—基础兽医学] S858.291[农业科学—兽医学]