新型抗菌肽Hadrurin基因的克隆与表达  

Cloning and Expression of Antimicrobial Peptide Hadrurin Gene

作　　者：曹琦[1] 黎满香[1] 刘珂[1] 颜运秋[1] 卢帅[1] 

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128

出　　处：《动物医学进展》2009年第4期43-46,共4页Progress In Veterinary Medicine

摘　　要：Hadrurin是一种分离自毒蝎的高活性抗菌肽,对多种细菌有较强抑制活性。本试验构建Ha-drurin全基因并在其两端加入了可切除的保护性多肽基因;再酶切Hadrurin基因产生黏性末端并连入pET-32a(+)原核表达载体;IPTG诱导表达抗菌肽蛋白,亲和层析纯化抗菌肽蛋白。结果表明,构建的Ha-drurin全基因片段序列完全正确,连接进入pET-32a(+)表达载体的基因由0.1 mmol/L终浓度的IPTG诱导5 h可达到表达的高峰,重组蛋白经亲和层析可到达纯化的目的。Hadrurin is a type of antimicrobial peptide which was isolated from the venom of the Mexican scorpion Hadrurus aztecus. Hadrurin demonstrates antimicrobial activity at low micromolar concentrations. In our research, Hadrurin gene was constructed by gene engineering method. Two protective fusion proteins were added. Hadrurin was induced by adding IPTG and purified by affinity chromatography. The results demonstrated that Hadrurin was correctly constructed and inserted into vector, 0.1 mmol/L final IPTG concentration and 5 h after induction could produce best expression in E. coil. Expression proteins were purified successfully by affinity chromatography.

关 键 词：Hadrurin抗菌肽 基因克隆 表达与纯化 

分 类 号：Q516[生物学—生物化学]