哺乳动物体细胞核移植的DNA甲基化重编程研究进展  

Progress on DNA Methylation Reprogramming of Somatic Cell Nuclear Transfer in Mammal

作　　者：沈彦军[1] 杨福合[1] 杜卫华[2] 朱化彬[2] 郝海生[2] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《动物医学进展》2009年第4期73-77,共5页Progress In Veterinary Medicine

基　　金：科技支撑计划肉牛项目(2006BAD14B08-04);国家自然基金项目(30700584);基本科研业务费项目“异种动物克隆技术的研究”

摘　　要：DNA甲基化是基因组主要的表观遗传修饰方式之一。核移植重构胚在对供体细胞基因组进行甲基化重编程过程中会出现异常的甲基化模式,而异常的甲基化重编程是导致克隆胚早期死亡及克隆动物发育畸形的主要原因。论文针对体细胞克隆动物基因组DNA的甲基化模式、造成克隆胚胎甲基化异常的原因及异常甲基化对重构胚胎发育的影响等进行了综述。深入研究核移植重构胚甲基化重编程的机制,有助于完善核移植技术,提高克隆效率,使其更好地应用于基础研究和生产实践。DNA metylation is one of the major epigenetic modifications. There will be abnormal DNA methylation patterns when the donor cell genome is methylation reprogrammed in reconstructed embryo, which might be the primary reasons for inducing premature death of cloned embryos and development abnormalities of cloned animals. The article discussed DNA metylation patterns of somatic cell nuclear transfer mammal, the reasons of inducing aberrant DNA methylation in cloned embryo, and the effect of the abnormal DNA methylation to nuclear transfer embryo development. Studying the precise mechanism of the DNA methylation reprogramming will be benefit for improving the technology and efficiency of somatic cell nuclear transfer, which will be eventually applied in the basic study and practice.

关 键 词：核移植 重编程 DNA甲基化 异常 

分 类 号：S814.8[农业科学—畜牧学]