高效液相色谱法测定祛痹舒肩丸中延胡索乙素含量  

Determination of dl-Tetrahydropalmatine in Qubi Shujian Pills by HPLC

作　　者：申强[1,2] 陈燕忠[1] 吕竹芬[1] 庄义修[3] 

机构地区：[1]广东省药物新剂型重点实验室.广东药学院药物研究所,广东广州510006 [2]长治医学院,山西长治046000 [3]广东食品药品职业学院,广东广州510520

出　　处：《中国药业》2009年第9期21-22,共2页China Pharmaceuticals

基　　金：广东省科技计划地市重点引导项目;项目编号:2006B35604008

摘　　要：目的建立测定祛痹舒肩丸中延胡索乙素含量的高效液相色谱(H PLC)法。方法色谱柱为Dikm a Diam onsilC18柱(250m m×4.6m m,5μm),流动相为0.1%磷酸溶液(用三乙胺调pH至4.0)-乙腈(80∶20),检测波长为280nm,流速为1.0m L/m in,柱温为室温。结果延胡索乙素进样量在0.0800～0.8000μg范围内与峰面积线性关系良好(r=0.9998,n=8),平均加样回收率为98.74%,RSD为1.94%(n=6)。结论H PLC法简便、灵敏、准确,可用于祛痹舒肩丸的质量控制。Objective To develop a HPLC method for determination of dl- tetrahydropalmatine in Qubi Shujian Pills. Methods The analysis was carried out on the Diamonsil C18 column （250 mm×4.6 mm, 5μm） with the mobile phase 0.1% phosphoric acid （adjusted to pH 4.0 by triethylamine）-acetonitrile （80：20）. The flow rate was 1.0 mL/min at the room temperature and the UV detection wavelength was 280 nm. Results The calibration curve was linear in the range of 0. 080 0-0. 800 0 μg for dl- tetrahydropalmatine （r =0. 999 8, n = 8）. The average recovery rate was 98.74% and RSD was 1.94% （n =6）. Conclusion The method is rapid, sensitive and accurate. It can be used for the quality control of this preparation.

关 键 词：高效液相色谱法 祛痹舒肩丸 延胡索乙素 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]