膦甲酸钠氯化钠注射液有关物质和含量的测定  

Determination of Foscarnel Sodium and Its Related Substances

作　　者：冮海峰[1] 邓岩辉[1] 孟祥军[1] 

机构地区：[1]沈阳医学院,辽宁沈阳110034

出　　处：《中国药业》2009年第9期32-33,共2页China Pharmaceuticals

摘　　要：目的建立测定膦甲酸钠氯化钠注射液有关物质和含量的方法。方法采用高效液相色谱(H PLC)法测定有关物质,固定相为聚合阴离子树脂(W atersIC PAK,5cm×4.6m m),流动相为邻苯二甲酸氢钾-1m ol/L硝酸溶液,检测波长为290nm,流速为1.4m L/m in,柱温为25℃;采用紫外分光光度(UV)法测定膦甲酸钠注射液的含量。结果膦甲酸钠质量浓度在0.24～0.36m g/m L时与吸收度具有良好的线性关系(r=0.9998,n=5),平均回收率为100.06%,RSD=0.95%(n=9)。结论所用方法简便、快速、准确,可用于膦甲酸钠氯化钠注射液有关物质和含量的测定。Objective To establish a method for the assay of foscarnel sodium and its related substances by HPLC. Methods The aggregating anion colophony column（Waters IC PAK,5 cm×4.6 mm）was used. The mobile phase was potassium hydrogen phthalate-nitric acid solution （1 mol/L）. The detection wavelength was set at 290 nm. The flow rate was 1.4 mL/min. The column temperature was 25 ℃. Results The linear range of foscarnel sodium was 0. 24-0.36 mg/mL （r =0. 999 8, n =5）. The average recovery rate was 100. 06%, RSD =0.95% （n =9）. Conclusion The method is simple, rapid and accurate. It can be used to determine the content of foscarnel sodium and its related substances in Foscarnel Sodium Injection.

关 键 词：膦甲酸钠 高效液相色谱法 有关物质 紫外分光光度法 

分 类 号：R927[医药卫生—药学] R978.7