HLA-DRB1^＊ 0701基因与吸烟在乙肝疫苗免疫应答中的交互作用  

Research on the interaction models of HLA-DRB1*0701 and smoking in the agents of Non-or hyporesponsse to hepatitis B vaccine

作　　者：黄晓晖[1] 陈思东[1] 

机构地区：[1]广东药学院公共卫生学院,广州510310

出　　处：《公共卫生与预防医学》2009年第2期7-10,共4页Journal of Public Health and Preventive Medicine

基　　金：广东省自然科学基金(020537)

摘　　要：目的探讨HLA-DRB1*0701基因与吸烟对乙肝疫苗无(弱)应答发生的交互作用及作用方式。方法选取1342名接种乙肝疫苗后无、弱应答者128人作为病例组,在有应答者中按性别匹配随机抽取128人作为对照组进行病例对照研究,对所有研究对象进行问卷调查,并应用PCR-SSP技术检测研究对象的HLA-DRB1*0701基因。采用SPSS13.0软件进行分析。结果HLA-DRB1*0701基因与无(弱)应答的发生有相关性;基因与吸烟对无(弱)应答的发生有交互作用,显著增加无(弱)应答的发生,且有明显的剂量反应关系;与开始吸烟年龄的交互作用呈低暴露—基因效应;与吸烟年限及每日吸烟量的交互作用呈高暴露—基因效应。结论HLA-DRB1*0701基因与吸烟对乙肝疫苗无(弱)应答的发生有交互作用。Objective To explore the interaction models between HLA-DRB1^＊ 0701 and smoking in the occurrence of non/or hyporesponsse to hepatitis B vaccine. Methods A case-control study including 128 non/or hyporesponders and 128 responders from 1342 healthy adults who were subjected to a standard course of immunization with recombinant hepatitits B virus vaccine. All subjects were investigated with a uniform questionnaire. HLA-DRB1^＊ 0701 genes were detected with ploymeme chain reaction-sequence specific primes（ PCR-SSP）. The data were analysed by SPSS 13.0 software. Results The result showed that the frequency of HLA-DRB1^＊ 0701 had significant difference both in the cases and controls. Both HLA- DRB1^＊ 0701 and smoking showed significant gene-environment interaction. Furthermore, interaction of the DRB1^＊ 0701 with the age of starting smoking showed a low exposure-gene（LEG） effect on non/or hyporesponse,and that of the DRB1^＊ 0701 and smoking years or smoking per day showed a high exposure-gene （HEG） effect. Conclusion HLA-DRB1^＊ 0701 had significant gene-environment interaction with smoking in the occurrence of non/or hyporesponsse to hepatitis B vaccine.

关 键 词：HLA-DRB1^＊0701 乙肝疫苗 无(弱)应答 吸烟 交互作用 

分 类 号：R186[医药卫生—流行病学] R512.62[医药卫生—公共卫生与预防医学]