酸性高锰酸钾体系荧光分光光度法测定多塞平新方法研究  

Fluorimetric Determination of Trace Doxepin by Potassium Permanganate-Sulfuric Acid New System

作　　者：张泾凯[1] 孙飞龙[1] 李建国[1] 屠一锋[1] 

机构地区：[1]苏州大学材料与化学化工学部

出　　处：《光谱实验室》2009年第2期444-446,共3页Chinese Journal of Spectroscopy Laboratory

基　　金：苏州大学博士科研资助基金(13109730);生命分析化学教育部重点实验室开放基金(KLACLS07016);江苏省高校自然科学研究计划项目(08KJB150014);苏州大学国家级重点学科有机化学培育点扶持学科科研启动基金的资助

摘　　要：硫酸介质中,多塞平可以还原高锰酸钾,生成强荧光产物,其在激发波长336nm,发射波长465nm处的荧光强度与多塞平浓度有良好的线性关系,拟定了测定多塞平的荧光分析新方法。该方法操作简便,灵敏度高,背景低,选择性好,测定多塞平的线性范围为0.1—40mg/L,检出限为0.026mg/L,用于药物中多塞平的测定,获得了令人满意的结果,并对反应机理进行了探讨。A novel fluorimetric method for the determination of trace amounts of doxepin has been proposed based on the reaction of doxepin with potassium permanganate in sulfuric acid medium,and forms a fluorescent product at excitation wavelength 336nm and emission wavelength of 465nm,respectively the linear relation between doxepin concentration with fluorescence intensity is in the ranges of doxepin 0.1—40mg/L,the detection limit is 0.026mg/L.The method is simple,rapid,sensitive and with good selectivity,and was used for the determination of trace amounts of doxepin in medicament samples with satisfactory results.The mechanism of the reaction was also discussed.

关 键 词：荧光分析 H2SO4-KMnO4体系 多塞平 药物分析 

分 类 号：O657.32[理学—分析化学]