准噶尔盆地大沙鼠鼠疫自然疫源地鼠疫耶尔森菌质粒谱的研究  

Study on the map of plasmid of Y. pestis in the R. opimus natural plague foci in Junggar Basin

作　　者：郭荣[1] 辛有全[2] 王信惠[1] 祁芝珍[2] 戴翔[1] 

机构地区：[1]新疆疾病预防控制中心,乌鲁木齐830002 [2]青海省地方病预防控制所

出　　处：《中华微生物学和免疫学杂志》2009年第4期291-293,共3页Chinese Journal of Microbiology and Immunology

摘　　要：目的了解和掌握准噶尔盆地大沙鼠鼠疫自然疫源地鼠疫菌质粒谱类型。方法提取新疆地区不同鼠疫自然疫源地及青藏高原喜玛拉雅旱獭鼠疫自然疫源地、昆仑山喜马拉雅旱獭鼠疫自然疫源地和内蒙古长爪沙鼠鼠疫自然疫源地的39株鼠疫菌质粒DNA，采用琼脂糖凝胶电泳法对其进行质粒谱图型分析。结果在检测的26株准噶尔盆地大沙鼠鼠疫自然疫源地鼠疫菌中，有23株质粒谱为6×10^6、45×10^6、65×10^6，3株为6×10^6、45×10^6和72×10^6。结论准噶尔盆地大沙鼠鼠疫自然疫源地鼠疫菌质粒谱分为两个类型：一为6×10^6、45×10^6、65×10^6质粒谱型，是该疫源地的主要质粒谱型，与邻近的北天山灰旱獭一长尾黄鼠鼠疫自然疫源地及内蒙古长爪沙鼠鼠疫自然疫源地、昆仑山喜马拉雅旱獭鼠疫自然疫源地和青藏高原喜马拉雅旱獭鼠疫自然疫源地的鼠疫菌主要质粒谱型一致；二为6×10^6、45×10^6和72×10^6质粒谱型，为少数型，在我国系首次发现。Objective To investigate the type of plasmid map of Y. pestis in the R. opimus natural plague foci in Junggar Basin. Methods A total of 39 plasmid DNA of Y. pestis which were isolated from the natural plague foci of Junggar Basin, Tianshan Mountain, Kunlun Mountain, Qinghai-Tibet Plateau and Inner Mongolia were extracted by the methods of Kado and Liu. The plasmid map was analyzed by the methods of agarose gel electrophoretogram. Results Two types of plasmid map were found in 26 Y. pestis which were isolated from Junggar Basin. Of them 23 were 6 ×10^6, 45 ×10^6 and 65×10^6 type of plasmid map, and 3 were 6 ×10^6 , 45×10^6 and 72×10^6type. Conclusion There are two types of plasmid map in the R. opimus natural plague foei in Junggar Basin. One type, which is the dominant type in this area, is 6 ×10^6 , 45 ×10^6 and 65×10^6 type. This type is also similar to the dominant plasmid map type of the nature plague foci of Tianshan Mountain, Kunlun Mountain, Qinghai-Tibet Plateau and Inner Mongolia. The other type is 6×10^6, 45 ×10^6 and 72×10^6type, and this type is new plasmid map type of Y. pestis in our country.

关 键 词：鼠疫耶尔森菌 质粒 准噶尔盆地 

分 类 号：R181.3[医药卫生—流行病学]