新疆伊犁地区马和驴博尔纳病病毒自然感染的调查  

Epidemiological investigation of Borna disease virus infection in horses and donkeys in Yili, Xinjiang

作　　者：张英英[1] 展群岭[1] 徐鸣明[1] 余建萍[1] 曾志磊[1] 翟红[1] 刘妍汐[1] 陈晓[1] 彭丹[1] 朱丹[1] 胡永波[1] 霍康[1] 谢鹏[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科,400016

出　　处：《中华微生物学和免疫学杂志》2009年第4期321-325,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家高技术研究发展计划（863计划）（2006AA02Z196）

摘　　要：目的调查新疆伊犁地区伊犁马和伊犁驴博尔纳病病毒（Borna disease virus，BDV）流行现状，分析BDV种系来源。方法采用荧光定量巢式实时逆转录聚合酶链反应（fluorescence quantitative nested RT—PCR，FQ—nRT—PCR），对新疆伊犁地区518匹伊犁马和206头伊犁驴外周血单个核细胞（peripheral blood mononuclear cell，PBMC）进行BDVp24基因片段检测。对检测阳性结果的标本通过BDVp40和质粒标准品的检测进行进一步验证并测序，对其进行基因同源性、氨基酸序列和系统发生分析。结果5例伊犁马和4例伊犁驴血标本BDVp24检测阳性，阳性率分别为0．97％和1．94％；9例标本BDVp40片段检测均阳性，质粒标准品检测均阴性；BDV024扩增产物序列与He／80株的同源性为100％。结论新疆伊犁地区伊犁马和伊犁驴中可能存在BDV的自然感染，该地区BDV流行株与He／80株存在同源性。Objective To investigate the epidemiology of BDV infection in Yili horses and Yili donkeys and to analyze phylogenetic source of BDV in Yili area, Xinjiang. Methods We established fluorescence quantitative nested RT-PCR to detect BDV p24 segment in peripheral blood mononuclear cells （PBMCs） of 518 Yili horses and 206 Yili donkeys in Yili area, Xinjiang. Positive products were validated by detecting BDV p40 segment and plasmid to preclude the contamination, and were sequenced to analyze the homology of gene sequence, amino acid sequence and phylogenetic tree. Results The positive rates of BDV infection in PBMCs of 518 Yili horses and 206 Yili donkeys were 0.97% and 1.94% , respectively. The results of BDV p40 segment verification were positive in all of the samples of BDV p24 positive. All the samples tested were not contaminated by plasmid. There was a homology of the gene sequence of positive PCR samples with strain He/80. And the gene sequence revealed more than 93% identical to H1766 and strain V. Conclusion Our study suggested BDV natural infection in Yili horses and Yili donkeys. The endemic BDV had a high degree of identity to strain He/80.

关 键 词：博尔纳病病毒 荧光定量巢式实时逆转录聚合酶链反应 TAQMAN探针 伊犁马 伊犁驴 

分 类 号：S858[农业科学—临床兽医学]