禽流感H5、H9亚型同步检测压电免疫芯片的研究  

Piezoelectric immunity protein chips for determination of H5,H9 subtype avian influenza viruses

作　　者：林向华[1,2] 刘仲明[1] 刘忠民[3] 

机构地区：[1]广州军区广州总医院医学实验科,广州510010 [2]中山大学附属第二医院检验科,广州510120 [3]广州医学院检验系,广州510182

出　　处：《中国卫生检验杂志》2009年第4期820-822,共3页Chinese Journal of Health Laboratory Technology

基　　金：广东省重大科技项目(2008A030203005)

摘　　要：目的：研究一种新型、快速的同步检测禽流感H5、H9亚型的压电免疫芯片。方法：以AT切型、基频10MHz的石英晶体为材料,设计四通道芯片微阵列。采用聚乙烯亚胺粘附-戊二醛交联法将禽流感H5、H9亚型单克隆抗体固定在石英晶体的金膜电极表面制备生物敏感膜,构建可用于同步检测禽流感H5、H9亚型的压电免疫芯片。结果：H5、H9亚型单克隆抗体的稀释度均为1：10时可使压电免疫芯片达到最佳的抗体固定量,H5亚型抗原稀释度在1：2-1：40范围内、H9亚型抗原稀释度在1：10-1：160范围内均呈现较好的线性关系。构建的压电免疫芯片用于检测H5、H9亚型抗原和疑似样品,其各自的响应值与空白对照的噪声值之比均大于2。结论：本次实验构建的压电免疫芯片可用于禽流感H5、H9亚型的定性检测。Objective：To establish rapid piezoelectric immunity protein chips for determination of H5,H9 subtype avian influenza viruses, Methods：HS, H9 subtype avian influenza virus monoclonal antibodies were deposited on the gold electrode＇s surface of 10 MHz quartz AT - cut crystal by polyethyleneimine adhesion and glutaraldehyde cross - linking method to make the chips. Results：The optimum condition of the immobility of antibodies was 1 ： 10 dilution, the linear range of the dilution for H5 antigen between 1：2 - 1 ：40, and of H9 antigen between 1 ： 10 - 1 ： 160. The H5, H9 antigens and the suspected samples were 2 times higher than the blank by using the chips. Conclusion：The chips can be used for qualitative detection.

关 键 词：禽流感 戊二醛 压电石英晶体 免疫传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]