用于检测尿液中HIV抗体的胶体金免疫层析快速诊断试纸条的制备  

Preparation of colloidal gold immunochromatographic test kit for quickly diagnosing HIV antibody in urine

作　　者：李晓娜[1,2] 赵晓阳[1,3] 郭亮[1] 王彪[1] 郝凤进[1] 关一夫[1] 

机构地区：[1]中国医科大学基础医学院生物化学与分子生物学教研室,沈阳110001 [2]中国医科大学基础医学院化学教研室,沈阳110001 [3]沈阳医学院基础医学院化学教研室,沈阳110001

出　　处：《中国卫生检验杂志》2009年第4期823-825,888,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的：制备一种可以通过检测尿液中HIV抗体来进行无创伤HIV感染的胶体金免疫层析快速诊断试纸条。方法：利用柠檬酸三钠还原法制备直径38 nm的胶体金溶液,利用HIV-1 gp41抗原标记胶体金,制备胶体金免疫复合物,组装胶体金免疫层析快速诊断试纸条。结果：胶体金颗粒粒径均一,胶体金免疫复合物性能稳定,特异性强,试纸条检测时间只需10-20 min。结论：胶体金免疫层析试纸条使用简便,检测快速,结果准确,无需特殊的仪器设备,性价比高,适用于基层医疗单位使用和对大量人群的规模筛查等。Objective：To develop the colloidal gold immunochromatographic test kit for quick, non - invasive diagnosis of HIV infection by testing HIV antibody in urine. Methods：Preparing the colloidal gold particles of 38 nm in diameter using a method of citrate trisodium, immobilizing HIV -1 gp41 antigen on colloidal gold particles, and assembling the test kit. Results： Colloidal gold particles are uniform; the colloidal gold immuno complex is stable with a strong specificity. Testing results can be obtained in 10 - 20 minutes. Conclusion ：The colloidal gold immunochromatographic test kit is convenient in use with fast testing speed and accurate result. The testing result can be read easily without any assistance of special apparatus and instruments. It has potential utility in fast medical diagnoses and quick screening of mass population.

关 键 词：HIV检测 胶体金免疫层析 尿液标本 快速检测 

分 类 号：R446.6[医药卫生—诊断学]