实时荧光PCR技术在肠道病毒71型检测中的应用  

Application of real-time fluorescent PCR for detection of enterovirus type 71

作　　者：甘莉萍[1] 陈应坚[1] 杨慧[1] 

机构地区：[1]广东省深圳市龙岗区疾病预防控制中心,广东深圳518172

出　　处：《中国卫生检验杂志》2009年第4期826-827,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:探讨实时荧光RT-PCR和逆转录聚合酶链反应(RT-PCR)检测肠道病毒EV71病毒核酸的应用。方法:采集2008年5月深圳市龙岗区幼儿疑似肠道病毒感染者粪便和疱疹液标本,分别用实时荧光RT-PCR方法和逆转录聚合酶链反应(RT-PCR)方法对肠道病毒EV71病毒进行检测。结果:在对16例已收集到的患者的粪便、疱疹液标本进行检测时,用实时荧光RT-PCR方法检测到8份阳性样本,阳性率为50.0%(8/16),而用逆转录聚合酶链反应(RT-PCR)方法检测到5份阳性样本,阳性率为31.3%(5/16)。结论:实时荧光RT-PCR技术具有特异性强,灵敏度高的特点,且能在采样后数小时内检测到病毒的RNA,从而达到快速诊断的目的,因此该方法对肠道病毒的流行病学调查及治疗起重要作用。Objective：To investigate the application of real - time fluorescent RT - PCR and reverse transcription polymerase chain reaction （RT -PCR） for the detection of Enterovirus type 71 nucleotide. Methods： Stool and herpes fluid specimens were collected from children with suspect Enterovirus infection in Longgang District of Shenzhen City in May 2008. Real - time fluorescent RT- PCR and RT- PCR were applied to the detection of EV71, respectively. Results：A total of 16 samples were collected from stool and herpes fluid specimens and were performed the detection of EV71. Eight of them were positive by real - time fluo- rescent RT- PCR, the positive percentage was 50. 0% （8/16）, while five were positive by RT -PCR, the positive percentage was 31.3% （5/16）. Conclusion：Real -time fluorescent RT - PCR is a highly specific and sensitive method and can detect virus RNA within several hours after specimen collections, so that it can rapidly diagnose virus DNA and plays an important role for the investigation of epidemiology and treatment.

关 键 词：肠道病毒EV71 实时荧光RT-PCR RT-PCR 

分 类 号：R373.2[医药卫生—病原生物学]