国产乙肝表面抗原金标试纸条检测性能的评价  

Quality and performance evaluation of gold immunochroma-tography strips for HBsAg detection

作　　者：蔡文品 吴可可[2] 

机构地区：[1]浙江省温州市第八人民医院检验科,浙江温州325000 [2]温州医学院,2008届实习生浙江温州325000

出　　处：《中国卫生检验杂志》2009年第4期846-847,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:对3种国产乙肝表面抗原(HBsAg)胶体金免疫层析法(GICA)试纸条检测性能做初步研究,明确HBsAg金标法检测在临床上的适用性。方法:用临床血标本对3种金标试纸条渗透性、均一性等物理现象作观察,对最低检测下限、特异性、重复性、后带现象进行初步研究。结果:3种G ICA试条对HBsAg的最小检出量可达2.5 IU/L;特异性100%;未发现后带现象。结论:金标法检测HBsAg适合于血站、口岸等的初筛,不宜用于临床上的快速检测。Objective：To investigate the quality and performance of 3 sources of gold immunochromatography assay （GICA） strips for HBsAg detection and to define the clinical applicability of the GICA strips. Methods ： A few patients blood samples were tested by immunoluminescence analyzer. The minimal detection limits, sp - ecificity, postzone phenomenon and physical properties of the strips were assessed. Results：The minimal detection limits of the 3 sources of GICA strips could reach 2. 5 IU/L. The specificity was 100%. No post phenomenon was found. Conelusion：GICA for HBsAg is suitable for screening test in port, blood -do- nate station etc. But it is not fit for clinical rapid detection.

关 键 词：胶体金免疫层析法 乙型肝炎病毒表面抗原 后带现象 

分 类 号：R446.5[医药卫生—诊断学]