常见革兰阴性杆菌的检出率及耐药性分析  

An analysis of common gram-negative bacilli detection rate and drug resistance

作　　者：孟丽[1] 尤佳女[1] 赵肖君[1] 汪强[1] 

机构地区：[1]浙江中医药大学附属第二医院检验科,杭州310005

出　　处：《中国卫生检验杂志》2009年第4期870-871,938,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：了解本医院常见革兰阴性杆菌的检出率和耐药性。方法：细菌鉴定使用ATB Expression系统鉴定,采用K-B法对临床分离菌株进行抗菌药物敏感试验,用双纸片扩散确诊法判定产ESBLs菌株。回顾分析了2004年1月-2008年10月临床送检标本的病原菌的鉴定和抗菌药物敏感试验资料。结果：革兰阴性杆菌是医院感染中的主要病原菌,而大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌、鲍曼不动杆菌是最常见的病原菌,总阳性率为44.54%。除亚胺培南和头孢哌酮-舒巴坦外,其他常用抗菌药物的耐药率均较高。结论：进行规范的病原菌培养和耐药监测,是临床医生合理使用抗菌药物的基础,对减少医院感染的发生和延缓耐药菌的产生具有重要意义。Objective： To investigate the detection rate and drug resistance of common Gran - negative bacilli in our hospital. Methods：Bacterial strains were identified by ATB Expression bacteria analysis system, and Antibiotic susceptibility tests were performed by Kirby - Bauer method. ESBL producing strains were detected by double - disc diffusion confirm test. The data of bacteria strains and antimicrobial susceptibility from January 2004 to October 2008 have been analyzed. Results： Gran - nega- tive bacteria is the principal pathogenic bacteria in hospital infection, and Escherichia cob, Klebsiella pneumonia, Pseudomonas aeruginosa, Acinetobaeter banmanni are the most common （44. 54% ）. The resistance rates of other antimicrobials are also high except imipenen and cefoperazone - sulbactem. Conclusions： Standardized bacterial straining and drug resistance monitoring is the basis of reasonable drug usage in clinic, and is important for reducing hospital infection and retarding the occurrence of drug resistant strains.

关 键 词：革兰阴性杆菌 医院感染 分离率 耐药性 

分 类 号：R446.5[医药卫生—诊断学]