沈阳地区193份腹泻患者副溶血弧菌感染情况分析  

Detection of Vibrio parahaemolyticus in patients with diarrhea in Shenyang

作　　者：董雪[1] 李继耀[1] 王冰[1] 李欣[1] 金东[2] 崔志刚[2] 景怀琦[2] 

机构地区：[1]沈阳市疾病预防控制中心,沈阳110031 [2]中国疾病预防控制中心传染病所,北京102206

出　　处：《中国卫生检验杂志》2009年第4期893-895,共3页Chinese Journal of Health Laboratory Technology

基　　金：沈阳市科技局资助项目(1063231-3-00)

摘　　要：目的:了解沈阳地区腹泻患者副溶血弧菌的感染情况。方法:本地医院送检的腹泻病人标本,经分离培养、生化鉴定、血清分型以及PCR技术检测毒力基因。结果:193份腹泻病人的标本中,检出副溶血弧菌43株,阳性率22.3%。43株副溶血弧菌共分4个血清群,其中O3,O1,O4居多,分别占60.5%,23.3%和14%。toxR基因检测均阳性,毒力基因分布以tdh阳性和trh阴性为主,占93%(40/43)。结论:沈阳地区副溶血弧菌以O3血清为主,tdh阳性和trh阴性为主要毒力基因型,tdh阴性和trh阳性(或阴性)较少。Objective： To survey the Vibrio parahaemolyticus in patients with diarrhea and study on the position of it in diarrhea. Methods： Various bacteria were isolated and identified from diarrhea samples of native hospital pathogenic through separate culture, biochemistry, serology and PCR tested. Results： Totally 193 shares of samples from diarrhea patients were tested. Among all samples ,43 strains were positive for Vibrio parahaemolyticus, the detectable rate was 22. 3%. 43 strains Vibrio parahaemolyt/cus belonging to 4 serotypes, 03,01, 04 were common serotypes, accounting for 60.5 %, 23. 3 % and 14% respectively. All strains were toxR positive. In 43 Vibrio parahaemolyticus isolates from diarrhea patients, strains with tdh - positive and trh - negative were detected with proportions of 93% （40/43）. Condusion：The most frequent serotypes of Vibrio parahaemolyticus in Shenyang is 03, the majority of Vibrio parahaemolyticus is tdh - positive and trh - negative. The tdh - negative and trh - positive （or negative）strains are found to be less.

关 键 词：副溶血弧菌 感染 分析 

分 类 号：R378.3[医药卫生—病原生物学]