酸性染料比色法测定天仙子及其炮制品总生物碱含量  

Contents Determination of Total Alkaloids in Seeds of Hyoseyamus niger L. and Their Processed Samples by Acid Dye Colorimetry

作　　者：杨丹[1] 李军 解世全[1] 门启鸣 熊英 

机构地区：[1]辽宁中医药大学药学院,大连116600 [2]大连市药品检验所,大连116021 [3]沈阳宜事达化工有限公司,沈阳110161

出　　处：《中国医药导刊》2009年第3期481-482,479,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:探讨天仙子总生物碱含量测定方法。方法:应用酸性染料比色法测定天仙子及其炮制品总生物碱含量。结果:硫酸阿托品在2.208μg·ml～13.248μg·(ml)^(-1)范围内星线性关系.加样回收率为98.41%,RSD为0.96%(n=5)。测得天仙子总生物碱含量生品为0.290mg·g^(-1),清炒品为0.242mg·g^(-1),醋炒品为0.420mg·g^(-1),醋煮品为0.548mg·g^(-1).炮制品中总生物碱含量以醋煮法最高。结论:本含量测定方法操作简单,灵敏度高,准确度好,重现性好。该实验为天仙子及其炮制品生物碱含量测定提供科学依据。Objective： to determine the content of total alkaloids of in seeds of Hyoscyamus niger L. and their processed samples. Methods： Acid dye cotorimetry was used for the determination, Results： with atropine sulfateline 2.208-13.248ug·ml^-1 in the linear range, the average recovery was 98.41%, RSD = 0.96% （n=5）. The total alkaloid content of the raw seeds was 0.290mg·g^-1, the stir-fried seeds 0.242mg·g^-1, the stir-fried with vinegar 0.420mg·g^-1, the boiled with vinegar 0.548mg·g^-1, among which the seeds boiled with vinegar possessed the highest content of total alkaloids： Conclusion： This method is simple, highly sensitive and reproducible. The experiment provides with a scientific basis for the determination of alkaloid content in raw and processed seeds of Hyoscyamus niger L.

关 键 词：酸性染料比色法 天仙子 总生物碱 炮制 

分 类 号：R286[医药卫生—中药学]