大豆F3’H基因植物表达载体的构建及拟南芥的遗传转化  

Construction of Plant Expression Vector and Transformation in Arabidopsis thaliana of F3’H of Soybean

作　　者：马键[1] 宋雯雯[1] 张颖[1] 韩雪[1] 田俊[1] 王继安[1] 

机构地区：[1]东北农业大学大豆生物学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《作物杂志》2009年第2期58-62,共5页Crops

基　　金：国家自然科学基金资助项目(30471092)

摘　　要：通过RT-PCR方法扩增了大豆F3’H基因并构建含大豆荚皮绒毛色基因的重组质粒pBI121/F3’H的植物表达载体,转化到根癌农杆菌LBA4404菌株中形成工程菌株,通过根癌农杆菌介导的方法转化拟南芥。为进一步研究大豆荚皮绒毛色基因(F3’H)作为植物基因工程的报告基因的可能性奠定基础。The F3＇H gene of soybean was amplified by using RT-PCR. A soybean-containing pubescence color gene of the recombinant ptasmid pBI121/F3＇H plant expression vector was constructed and transformed into Agrobacterium LBA4404 as the formation of engineering strain. Then Agrobacterium LBA4404 transformed into Arabidopsis thaliana by infection.

关 键 词：大豆 根癌农杆菌 F3’H基因 植物表达载体 

分 类 号：S565.1[农业科学—作物学]