稳定大鼠原位肝移植模型的建立  

The establishment the rat stable model of orthotopic liver transplantation

作　　者：张安红[1] 李元栋[2] 邹浩军 徐钧[4] 

机构地区：[1]山西医科大学第一临床医学院普通外科,山西太原030001 [2]华中科技大学同济医学院附属协和医院胰腺外科,湖北武汉430022 [3]长治市第二人民医院普外科,山西长治046000 [4]山西省人民医院普外科,山西太原030012

出　　处：《中国医药导报》2009年第13期33-35,共3页China Medical Herald

基　　金：2007年回国留学科研资助项目(99)

摘　　要：目的:建立稳定大鼠原位肝移植模型,探讨其方法与技术。方法:用改进的二袖套法行140例SD→LEW大鼠原位肝移植,术中门静脉、肝下下腔静脉用袖套法吻合,肝上下腔静脉用缝合法吻合,胆管内支架法完成胆道重建。结果:供体手术时间为(27.0±3.1)min;供肝修整时间为(11.0±2.1)min;受体手术时间为(30.0±2.5)min;无肝期为(15.0±2.6)min。手术成功率(术后存活2d以上)为87%,1周存活率为81%,1个月存活率为72%。结论:提高手术技巧,熟悉各种并发症的原因和预防处理措施,可以减少并发症的发生,提高手术成功率,延长大鼠术后存活时间。Objective： To establish the rat stable model of orthotopic liver transplantation, and explore the methods and technology. Methods： 140 cases of SD→LEW rat liver transplantation had been done with improved two-cuff method in the portal vein, hepatic inferior vena eava under the law in line with cuff, liver superior and inferior vena cava was sutured with suture line. Biliary tract reconstruction was completed in bile duct stent law. Results： Donor surgery time was （27.0± 3.1） min; liver repaired time was （11.0±2.1） min; surgery receptor time was （30.0±2.5） min; no liver time was （15.0±2.6） min. The success rate （survived more than 2 days after operation） was 87%. One week survival rate was 81%, one month survival rate was 72%. Conclusion： Enhancing the surgery skill and understanding the reasons and prevention and treatments of each kinds of complication may reduce the occurrence of complications, raise the surgery success rate and lengthen the survivaltime of rats.

关 键 词：大鼠 肝移植 动物模型 手术技巧 

分 类 号：R65[医药卫生—外科学]