虎源猫泛白细胞减少症病毒灭活疫苗的制备及初步应用  

Preparation of the vaccine with inactivated Feline Panleukopenia Virus isolated from tiger and the preliminary application

作　　者：于亚丽[1] 周明[2] 张进[1] 华育平[1] 王立刚[2] 刘熠楠[1] 刘丹[2] 夏咸柱[3] 

机构地区：[1]东北林业大学野生动物资源学院,哈尔滨150040 [2]黑龙江东北虎林园,哈尔滨150028 [3]军事医学科学院军事兽医研究所,长春130062

出　　处：《微生物学报》2009年第5期643-647,共5页Acta Microbiologica Sinica

基　　金：国家林业局野生动植物保护管理项目资助~~

摘　　要：【目的】研制出虎源猫泛白细胞减少症病毒灭活疫苗并对其应用效果进行评价。【方法】以FPV-HLJ为种毒,按培养液体积1/10(V/V)的接毒量,采用同步接毒的方法接于猫肾传代细胞系F81株,于37℃静置培养,待细胞病变(CPE)达到75%以上时,进行收毒。病毒悬液经甲醛灭活24h,加入佐剂氢氧化铝胶,制备成FPV-HLJ细胞培养灭活疫苗。将灭活苗皮下接种2月龄非免疫家猫,确定其能产生较好的保护后,将该苗接种2月龄左右幼虎。【结果】实验组免疫猫FPVHI抗体水平随免疫次数的增加而呈上升趋势,三免后FPVHI抗体效价为1∶1024～1∶2048,且免疫猫能抵抗FPV强毒攻击,具有100%的存活率。幼虎三免后抗体效价多数能达到1∶1024。【结论】表明此灭活苗可产生较为理想的免疫效果。[ Objective] To prepare a vaccine with inactivated Feline Panleukopenia Virus （FPV） isolated from Siberian tigers and to evaluate its immunological effect. [ Methods] FPV-HLJ, an FPV strain previously isolated from Siberian tiger in our lab was used to inoculate cat kidney cell line FS1 with dose 1/10（v/v） using the synchronizing inoculation method. Inoculated F81 cell line was cultured at 37 ~C and collected when cytopathic effect was up to 75 %. Viral suspension was inactivated by using formaldehyde for 24 h and inactive vaccine preapred by adding aluminium hydroxide gel as adjuvant to the suspension. The inactive vaccine was applied to 2- month old kitten tigers of hypodermically after protection effect was proved in 2-month old nonimmunized cats by using the same vaccination procedure. [ Results] Antibody level in vaccinated cats revealed an increasing trend that the valence of antibody reached 1 ： 1024 - 2048 after three times of vaccination. All vaccinated cats survived challenges of virulent FPV virus. The valence of antibody reached 1 ： 1024 in most vaccinated tigers 15 days after the third vaccination. [ Conclusion] The results indicated that the inactivated vaccine can produce immunoprotection for tigers.

关 键 词：虎 猫泛白细胞减少症病毒 灭活疫苗 抗体检测 

分 类 号：S852.65[农业科学—基础兽医学]