类人胶原蛋白-透明质酸血管支架的性能及生物相容性  

Characterization and biocompatibility of human-like collagen-hyaluronic acid scaffold for blood vessel

作　　者：孙秀娟[1,2] 范代娣[1,1] 朱晨辉[1,1] 马晓轩[1] 骆艳娥[1] 陈岚[2] 郭佳庆[1] 

机构地区：[1]西北大学陕西省可降解生物医药材料重点实验室,西安710069 [2]西北大学生命科学院,西安710069

出　　处：《生物工程学报》2009年第4期591-598,共8页Chinese Journal of Biotechnology

基　　金：国家自然科学基金项目(No.20776119);陕西省教育厅专项科研计划项目(No.08JK452);西安市科技计划项目(No.YF07078)资助~~

摘　　要：将类人胶原蛋白与透明质酸按不同比例复合,控制透明质酸的终浓度(W/V)分别为0、0.01%、0.05%、0.1%,用京尼平交联,采用真空冷冻干燥方法构建出血管支架材料。通过扫描电镜、XPS分析、拉力测试、压力爆破实验、细胞毒性实验、血管支架细胞种植实验及小鼠皮下植入等方法对其表面超微结构、表面元素组成、力学性能、细胞毒性等级、细胞相容性、组织相容性进行了研究。结果表明:当透明质酸的含量为0.05%时,类人胶原蛋白-透明质酸支架的孔径比较均匀,孔隙率达94.38%,应力为(1000.8±7.9)kPa,爆破压力为(1058.6±8.2)kPa,细胞毒性实验合格,同时具有良好的细胞相容性、组织相容性及降解性能。Human-like collagen （HLC） was cross-linked with hyaluronic acid by genipin in different ratio. The concentrations of hyaluronic acid in the mixture were 0, 0.01%, 0.05% and 0.1%. The blood vessel tubular grafts were then fabricated by freeze-drying. Microstructure, element composite, mechanical properties, cytotoxicity grade, and biocompatibility of different vascular scaffold groups were studied by scanning electron microscope （SEM）, X-ray photoelectron spectroscopy （XPS）, tensile test, burst pressure experiment, cytotoxicity experiment, endothelial cells planted in blood vessel scaffolds and hypodermic embedding of mice. The results showed that HLC-HA （0.05%） tubular scaffold exhibited interconnected well-distributed and porous structure and porosity of 94.38%; achieved the desirable mechanical property with stress of （1000.8±7.9） kPa and burst pressure of （1058.6±8.2） kPa, hypocytotoxicity, favourable cytocompatibility, hisocompatibility and disposition of degradation.

关 键 词：类人胶原蛋白 透明质酸 血管支架 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程]