山羊痘病毒TK基因的克隆与序列分析  

Cloning and Sequence Analysis of Thymidine kinase Gene from Goat Poxvirus

作　　者：罗阿东[1] 唐源[1] 文明[1,2] 岳筠[1] 程振涛[1] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]贵州大学动物疫病研究所,贵州贵阳550025

出　　处：《山地农业生物学报》2009年第2期141-145,共5页Journal of Mountain Agriculture and Biology

基　　金：贵州省优秀科技教育人才省长专项基金项目资助[黔省专合字(2006)12号];国家教育部科技重点项目资助(206132)

摘　　要：设计特异性引物并应用PCR方法扩增山羊痘病毒QL、LD、Y、B株TK基因序列片段,将其克隆至pMD18-T载体,构建重组质粒pMD18-T-TK。再将该重组质粒转化DH5α感受态细胞进行培养,对通过PCR、酶切鉴定为阳性的重组质粒进行序列测定。TK基因核苷酸和氨基酸同源性分析表明,4株测序毒株TK基因与参考毒株的同源性均为96.6%-100%,说明羊痘病毒TK基因具有高度的保守性。A pair of specific primers were designed to amplify the Thymidine kinase （TK） gene of goat poxvirus QL, LD, Y and B strains, and the PCR products were ligated into the pMD18 - T vector. The recombinant plasmids pMD18 -T- TK were transformed into DH5α competent cells. The positive recombinant plasmids were identified via PCR and restriction enzyme digestion, and consequently sequenced. Similarity analysis showed that the four strain shared 96.6% - 100% identity with the reference strains in nucleotide and amino acid levels, indicating that TK gene is very conservative .

关 键 词：山羊痘病毒 TK基因 克隆 序列分析 

分 类 号：S858.27[农业科学—临床兽医学] Q78[农业科学—兽医学]