时间分辨荧光免疫分析在抗-HBs检测中的应用  

Value of time-resolved fluorescence immunoassay in detection of HBsAb

作　　者：杜为强[1] 陈小桃[2] 曾钊宇[1] 

机构地区：[1]广东佛山市高明区人民医院检验科,528500 [2]广东佛山市高明区人民医院内二科,528500

出　　处：《检验医学与临床》2009年第9期668-669,共2页Laboratory Medicine and Clinic

摘　　要：目的探讨时间分辨荧光免疫分析(TrFIA)检测乙型肝炎病毒表面抗体(抗-HBs)的优点及临床价值。方法采用酶联免疫吸附试验(ELISA)和TrFIA分别检测262份血清标本抗-HBs,用Excel2003进行数据统计和分析,用Kappa一致性检验和u检验评估两种方法检测结果的一致性。结果ELISA检测抗-HBs阳性率53.8%(141/262),TrFIA检测抗-HBs阳性率56.9%(149/262),两者符合率为90.8%,经Kappa一致性检验K值为0.815,一致性强度较高;经u检验P<0.05,两种测定方法结果无统计学差异;TrFIA定量分析结果0～10U/L为43.13%,10～100U/L为27.86%,大于100U/L为29.01%。结论TrFIA是抗-HBs定量测定的一种较好方法,特别是对检测低滴度区间的抗-HBs。可以区别ELISA检测抗-HBs阳性人群中对乙型肝炎病毒的弱免疫力和有效免疫力。Objective To discuss the advantages and clinical value of detection of hepatitis B surface antibody （HBsAb）by time-resolved fluorescence immunoassay（TrFIA）. Methods HBsAb was detected in 262 serum samples by ELISA and TrFIA, using EXCEL 2003 to finish statistics and data analysis, assessing the consistency of detection results with Kappa and u test. Results The positive rate of HBsAb was 53.8%（141/262） by ELISA and 56.9% （149/262） by TrFIA. The consistency rate was 90.8%,K-value was 0. 815 with Kappa test. Consistency was high. The two methods results had no significant difference（P〈0.05） with u test. The quantitative analysis results by TrFIA were respectively 43.13%（0-10 U/L） ,27.86%（10-100 U/L） and 29.01%（〉100 U / L）. Conclusion TrFIA is a good method of determining HBsAb, especially for the detection of low titer HBsAb. It may distinguish weak immunity and effective immunity for hepatitis B virus in crowd with HBsAb positive by ELISA test.

关 键 词：时间分辨荧光免疫分析 乙型肝炎病毒表面抗体 酶联免疫吸附试验 

分 类 号：R185.5[医药卫生—流行病学]