浅低温联合七氟烷对大鼠海马脑片氧糖缺失损伤的影响  

Effects of combination of mild hypothermia and sevoflurane on oxygen-glucose deprivation injury in rat hippocampal slices

作　　者：王芳[2] 王志萍[1] 江山[1] 谢蕾[2] 

机构地区：[1]徐州医学院江苏省麻醉学重点实验室,221002 [2]徐州医学院附属医院麻醉科

出　　处：《中华麻醉学杂志》2009年第4期372-375,共4页Chinese Journal of Anesthesiology

基　　金：基金项目：江苏省卫生厅资助项目（H200723）;江苏省教育厅重大课题（08KJA320001）

摘　　要：目的探讨浅低温联合七氟烷对大鼠海马脑片氧糖缺失损伤的影响。方法雄性SD大鼠，体重80～100g，断头处死，剥离海马，制备脑片。取符合标准的海马脑片32片，随机分为4组（n=8）：氧糖缺失组（OGD组）、七氟烷组（Sev组）、浅低温组（MH组）和浅低温＋七氟烷组（MH＋Sev组）。OGD组和MH组分别于37℃或32℃下用经95％N2-5％CO2混合气体饱和的无糖（氧糖缺失）人工脑脊液（ACSF）灌流脑片14min；Sev组和MH＋Sev组分别于37℃或32℃下用预先经4％七氟烷平衡的有氧有糖ACSF灌流脑片14min，再用预先经4％七氟烷平衡的氧糖缺失ACSF灌流脑片14min。记录海马CA1区缺氧到复氧1h期间的顺向群峰电位（OPS）。采用比色法测定乳酸脱氢酶（LDH）释放率。结果与OGD组比较，Sev组和MH组OPS消失时间延长，OPS恢复程度升高，MH组LDH释放率降低（P〈0．05或0．01）；与MH组比较，MH＋Sev组OPS消失时间延长，OPS恢复程度和恢复率升高，LDH释放率降低（P〈0．05或0．01）；与Sev组比较，MH＋Sev组OPS消失时间延长，OPS恢复程度和恢复率升高，LDH释放率降低（P〈0．05或0．01）。结论浅低温（32℃）联合4％七氟烷可减轻大鼠海马脑片氧糖缺失损伤。Objective To investigate the effects of mild hypothermia combined with sevoflurane on oxygen-glucose deprivation injury in rat hippocampal slices. Methods Male SD rats weighing 80-100 g were anesthetized with ether and decapitated. The hippocampus were removed and sagittally sliced （400 μm thick） and placed in artificial cerebrospinal fluid （ACSF） aerated with 95 % O2-5 % CO2 . Thirty-two hippoeampal slices were randomly divided into 4 groups （ n = 8 each） ： group Ⅰ OGD ; group Ⅱ sevoflurane （ Sev ） ; group m mild hypothermia（MH） and group Ⅳ MH ＋ Sev. In group Ⅰ and Ⅲ, the hippoeampal slices were perfused with glucose-free ACSF and aerated with 95 % N2-5 % CO2 for 14 min at 370C and 32℃ respectively. In group Ⅱ and Ⅳ, the hippocampal slices were perfused with oxygenated, glucose-containing ACSF saturated with 4% sevoflurane for 14 min, then perfused with oxygen/glucose-deficient ACSF saturated with 4% sevoflurane at 37℃ and 32℃ respectively. Electrophysiologieal technique was used to record the amplitude of orthodromic population spike （OPS） in the stratum pyramidale of CA1 region during OGD and 1 h reoxygenation. The release rate of LDH was determined using chromatometry. Results Compared with group Ⅰ , the disappearance time of OPS was significantly prolonged, the recovery amplitude of OPS was significantly increased in group Ⅱ and Ⅲ and the release rate of LDH was significantly decreased in group Ⅲ （ P 〈 0.05 or 0.01）. Compared with group Ⅲ , the disappearance time of OPS was significantly prolonged, the recovery amplitude and rate of OPS were significantly increased and the release rate of LDH was significantly decreased in group Ⅳ （ P 〈 0.05 or 0.01 ）. Compared with group Ⅱ , the disappearance time of OPS was significantly prolonged, the recovery amplitude and rate of OPS were significantly increased and the release rate of LDH was significantly decreased in group Ⅳ （ P 〈 0.05 or 0.01 ）. Conclusion Combination of mild hypothe

关 键 词：低温 人工 麻醉药 吸入 缺氧 海马 

分 类 号：R96[医药卫生—药理学]