内皮祖细胞复合膀胱无细胞基质的生物相容性  

In vitro research on the biocompatibility of endothelial progenitor cells combined with bladder acellular matrix

作　　者：陈柏松[1] 张胜利[1] 耿红全[1] 潘骏[1] 吴少峰[1] 陈方[1] 

机构地区：[1]上海交通大学医学院附属新华医院泌尿外科,200092

出　　处：《中华实验外科杂志》2009年第5期624-626,F0003,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30672108）;上海交通大学医学院博士点建设基金项目（BXJ0725）

摘　　要：目的应用内皮祖细胞复合膀胱无细胞基质构建组织工程学膀胱。方法分离培养猪外周血内皮祖细胞，制备膀胱无细胞基质。体外检测细胞与膀胱无细胞基质的生物相容性。观察细胞与膀胱无细胞基质的体外复合。结果成功分离培养猪外周血内皮祖细胞，其表面标志分别为CD13325．1％、CD34 55．9％、flk-197．7％、CD31 82．0％、CD144 95．4％。成功制备膀胱无细胞基质。生物相容性检测证明膀胱无细胞基质对细胞无明显的细胞毒性，细胞活力保持在90％以上。体外观察细胞可以复合于无细胞基质，并在其上增殖、生长。结论可以应用内皮祖细胞作为种子细胞，膀胱无细胞基质作为支架材料构建组织工程学膀胱，可能成为提高体内组织工程膀胱血管化的一种新方法。Objective By using endothelial progenitor cells combined with bladder acellular matrix to construct a tissue engineered bladder. Methods Porcine peripheral endothelial progenitor cells were isolated and cultured, and the bladder acellular matrix was prepared. The biocompatibility of endothelial progenitor cells and bladder acellular matrix was detected in vitro. The endothelial progenitor cells cocombined with the bladder acellular matrix were observed in vitro. Results Endothelial progenitor cells were isolated and cultured successfully, and the cell markers included CD133 （25.1%）, CD34 （55.9%） ,ilk-1 （97.7%） ,CD31 （82.0%） ,CD144 （95.4%）. The bladder acellular matrix was prepared. It was proven that the bladder acellular matrix had no significant cytotoxicity, and the cell vitality was more than 90%. Endothelial progenitor cells could proliferate and grow on the bladder acellular matrix in vitro. Conclusion Endothelial progenitor cells can serve as seeded cells, and bladder acellular matrix as a scaffold to construct tissue engineered bladder, which may offer a possible new approach to increase the vascularization in the tissue engineered bladder.

关 键 词：内皮祖细胞 组织工程 膀胱 生物相容性 血管化 

分 类 号：R318.08[医药卫生—生物医学工程]