染料木黄酮对3T3-L1前脂肪细胞分化的作用及其机制研究  

EFFECT OF GENISTEIN ON ADIPOGENESIS OF 3T3-L1 PREADIPOCYTES AND ITS MECHANISM

作　　者：张萌[1] 池田克己 家森幸男 

机构地区：[1]中国天津中医药大学中药学院,天津300193 [2]日本武库川女子大学生活环境学部,日本西宫市663-8179

出　　处：《营养学报》2009年第2期152-155,共4页Acta Nutrimenta Sinica

基　　金：笹川医学奖学金资助

摘　　要：目的观察染料木黄酮(genistein,GEN)对3T3-L1前脂肪细胞分化的影响,探讨GEN抑制3T3-L1细胞分化的作用及分子机制。方法用含1-甲基-3-异丁基黄嘌呤、地塞米松和胰岛素的培养液(MDI)诱导3T3-L1前脂肪细胞分化,同时用GEN或p38抑制剂SB203580干预。作用6d后,用油红染色实验观察脂肪形成情况;检测细胞培养液中非酯化脂肪酸(NEFA)、甘油三酯(TG)的含量;用Western blotting技术检测细胞中脂肪酸合酶(FAS)的蛋白表达。用GEN预处理30min后,用胰岛素刺激3T3-L1细胞,检测磷酸化p38MAPK(p-p38)的蛋白表达。结果GEN可以有效抑制3T3-L1细胞的脂肪形成,降低培养液中NEFA、TG的含量。胰岛素刺激后,3T3-L1细胞中p-p38的表达迅速增强,并在5min时达到高峰,GEN可以有效降低p-p38的表达。GEN、SB203580均能降低FAS的蛋白表达。结论染料木黄酮能有效抑制3T3-L1前脂肪细胞的脂肪分化,其机制是通过p38途径对FAS的抑制发挥作用。Objective To investigate the effect and underling mechanism of genistein （GEN） on adipogenesis in 3T3-L1 preadipocytes. Method 3T3-L1 preadipocytes were induced to differentiate in the presence of vehicle （MDI）, GEN or SB203580. On D 6 after induction of differentiation, lipid accumulation was assessed using the dye Oil Red O, medium was collected and assayed for the content of NEFA and TG, and fatty acid synthase （FAS） expression was analyzed by Western blotting. After pretreatment with GEN for 30 min, 3T3-L1 preadipocytes were stimulated with insulin for 5 or 10 min. The phosphorylation of p38 MAPK （p38） was analyzed by Western blotting. Results GEN inhibited lipid accumulation and decreased NEFA and TG content of 3T3-L1 on D 6 after the induction of differentiation. Pretreatment with GEN inhibited phosphorylation of p38 when stimulated with insulin. Furthermore, GEN inhibited the expression of FAS. SB203580, a p38 inhibitor, mimicked the FAS inhibition effect of genistein, which suggested that the inhibitory effect of GEN on FAS was partially via the p38 pathway. Conclusion Geinstein attenuates the differentiation of 3T3-L1 via the inhibition of p38 and FAS.

关 键 词：染料木黄酮 3T3-L1前脂肪细胞 脂肪分化 磷酸化P38MAPK 脂肪酸合酶 

分 类 号：R589.2[医药卫生—内分泌]