祛瘀化浊法对脂肪肝大鼠P_(450)ⅡE1表达的影响  

Effects of Quyuhuazhuo Method on the Expression of Hepatocyte Cytochrome P_(450)ⅡE1 in Rats with Fatty Liver

作　　者：潘智敏[1] 马丽[2] 周飞[2] 

机构地区：[1]浙江省中医院,杭州310006 [2]浙江中医药大学,杭州310053

出　　处：《中国中医急症》2009年第5期777-779,共3页Journal of Emergency in Traditional Chinese Medicine

摘　　要：目的研究祛瘀化浊法对脂肪肝大鼠P450ⅡE1表达的影响。方法将52只Wistar大鼠于实验室适应性喂养1周后,随机分为空白对照组、脂肪肝模型对照组、易善复治疗组、调脂积冲剂治疗组、中药六味地黄丸治疗组。采用酒精合并高脂乳剂建立大鼠脂肪肝模型,以易善复和六味地黄丸为阳性对照,测定血清TC、TG、HDL-C、LDL-C、ALT、GGT、细胞色素P450ⅡE1(CYP2E1)免疫组化表达水平、肝重、肝脏系数和组织学检查。结果模型组大鼠肝组织出现明显的脂肪变,其血清中TC、TG、LDL-C、ALT、GGT、肝指数均高于正常组,HDL-C低于正常组。调脂积冲剂能降低肝指数,降低TC、TG、ALT、GGT、TC、TG、LDL-C的含量,升高HDL-C的含量,抑制CYP2E1的表达。结论祛瘀化浊法可降低CYP2E1的表达,能通过抗脂质过氧化减轻实验大鼠脂肪肝变性。Objective： To study the effect of Quyuhuazhuo Method on the expression of hepatocyte cytochrome P450ⅡE1 in rats with fatty liver. Methods： A total of 52 Wistar rats, after the fed adaptive control for a week in the laboratory, were randomly divided into blank group, model group, essentiale group, Tiaozhiji Medicinal Granule group, Liuweidihuang Pill group. The fatty liver models of rats were established by alcohol and high fat diet. The Tiaozhiji Medicinal Granule group and Liuweidihuang Pill group were set as positive control group. The serum levels of TC, TG, HDL-C, LDL-C, ALT, GGT, and the expression of the cytochrome P450 ⅡE1 （CYP2E1）, liver weight, liver coefficient and histological examination were recorded. Results： The liver tissue of the model group rats appeared the fat denaturation. The serum levels of TC, TG, LDL - C, ALT, GGT, and liver coefficient of model group were higher, and HDL - C was lower than blank group. Tiaozhiji Medicinal Granule could reduce liver coefficient and the content of TC , TG, ALT, GGT, LDL- C, increase the content of HDL - C, and inhibit the expression of the cytochrome P450ⅡE1. Conclusion： The Quyuhnazhuo Method can inhibit the expression of the cytochrome P450ⅡE1 and relieve the fat denaturation by anti lipid peroxidation.

关 键 词：祛痰化浊 脂肪肝 细胞色素P450 免疫组化 大鼠 

分 类 号：R285.5[医药卫生—中药学]