胶体金免疫层析法快速检测组织样品中喹诺酮类药物残留  

RAPID DETECTION OF THE QUINOLONES RESIDUE IN TISSUE THROUGH COLLOIDAL GOLD IMMUNOCHROMATOGRAPHIC ASSAY

作　　者：刘烜[1] 郑文杰[1] 贺艳[1] 赵卫东[1] 桑丽雅 张少恩 

机构地区：[1]天津出入境检验检疫局,天津300201 [2]杭州南开日新生物技术有限公司,江苏杭州310022

出　　处：《食品研究与开发》2009年第5期131-134,共4页Food Research and Development

摘　　要：为建立用于快速检测组织样品中喹诺酮类药物残留的胶体金免疫层析试剂,采用免疫竞争法,将抗喹诺酮类单克隆抗体-胶体金复合物包被在胶体金结合垫上,并将人工合成的喹诺酮类抗原包被在硝酸纤维素薄膜表面作为检测线(T线),其与待测样品中相应喹诺酮类药物竞争结合胶体金标记的抗喹诺酮类单克隆抗体,并能以颜色直观显示检测的定性结果。检测肉、鱼、虾等组织试样时,灵敏度最低值可达到10ng/mL,只需3min～5min,与类似物无交叉反应。A rapid, simple colloidal gold immunochromatographic assay for detecting quinolones （QNs）residues has been established. Nanocolloidal gold particles were prepared and labeled to an anti-quinolones monoelonal antibody. QNs was conjugated to bovine serum albumin （BSA） and dispersed on a nitrocellulose （NC） membrane to be the test line （T）. The more analyte present in the sample, the more effectively it would compete with the QNs-BSA for binding to the limited amount of gold-labeled anti- quinolones monoclonal antibody. The presence or absence of a colored band on the test line indicates a negative or positive result. When measuring the water sample spiked with quinolones, the minimum detection concentration can reach 10 ng/mL. The major advantage of the one-step strip test is that the test can be completed within 5 minutes. No cross-reaction occurred between quinolones and other veterinary drugs.

关 键 词：喹诺酮 半抗原 免疫测定 胶体金 

分 类 号：S859.84[农业科学—临床兽医学]