缺血后处理对家兔缺血-再灌注心功能和MDA、SOD的影响  

The Effects of Ischemic Postconditioning on Cardiac Function and Serum SOD,MDA during Ischemia-reperfusion of Myocardium in Rabbits

作　　者：彭艳玲[1] 刘兴德[1] 孙传聪[1] 

机构地区：[1]贵阳医学院附院心内科,贵州贵阳550004

出　　处：《贵阳医学院学报》2009年第2期137-140,共4页Journal of Guiyang Medical College

基　　金：贵州省高层次人才科研条件特助经费项目[TZJF(2006)12号]。

摘　　要：目的:研究缺血后处理对家兔心肌缺血-再灌注心功能、丙二醛(MDA)和超氧化物歧化酶(SOD)的影响。方法:24只新西兰大白兔随机分为3组,假手术组(A组)、缺血-再灌注组(B组)、缺血后处理组(C组);记录家兔心肌缺血前、缺血30 min,再灌注180 min时心脏血流动力学指标,检测家兔血清MDA、SOD的变化。结果:(1)与B组比较,C组再灌注180 min左室收缩压、左室内压最大收缩和舒张变化速率均显著升高(P<0.05),左室舒张末压显著下降(P<0.05);(2)与A组比较,B组血清中MDA含量显著增高(P<0.01),而SOD活性显著降低(P<0.01);与B组比较,C组血清中MDA含量显著降低(P<0.01),而SOD活性显著增高(P<0.05)。结论:缺血后处理能改善家兔缺血-再灌注心功能,其机制可能与减少自由基损伤和抗氧化作用有关。Objective： To investigate the effects of ischemic postconditioning on cardiac function and serum SO,MDA during ischemia-reperfusion of myocardium in rabbits.Methods： Twenty-four New Zealand white rabbits were divided randomly into 3 groups（8 in each）： Sham-operated group（A）,ischemia-reperfusion group（B）,and ischemic postconditioning group（C）.Cardiac function were evaluated with cardiac hemodynamic indexes;SOD and MDA in serum were assayed.Results： Compared with group B,left ventricular systolic pressure（LVSP） and maximal changing rate of left ventricular inner pressure（±dp /dtmax） increased remarkably in group C in 180 min after reperfusion（P〈0.05）,and left ventricular end-diastolic volume（LVEDP） decreased significantly（P〈0.05）;Serum MDA level in group B was significantly higher（P〈0.01）,while SOD level was significantly lower than those in group A（P〈0.01）;In group C,MDA level was significantly lower（P〈0.01） but SOD level was higher than those of group B（P〈0.05）.Conclusion： Ischemic postconditioning could improve cardiac function during ischemia-reperfusion injury in rabbits,of which the potential mechanism might involve anti-oxidization and reduction of free radical caused injury.

关 键 词：缺血后处理 心脏 心肌再灌注损伤 心室功能 左 丙二醛 超氧化物歧化酶 兔 

分 类 号：R541.41[医药卫生—心血管疾病]