钙和钙调素对机械刺激诱导的烟草悬浮培养细胞耐热性的调控  

Involvement of Calcium and Calmodulin in the Mechanical Stimulation-induced Heat Tolerance in Tobacco(Nicotiana tabacum L.) Suspension Culture Cells

作　　者：李忠光[1] 龚明[1] 

机构地区：[1]云南师范大学生命科学学院,昆明650092

出　　处：《植物生理学通讯》2009年第4期363-366,共4页Plant Physiology Communications

基　　金：国家自然科学基金(30460016);云南省自然科学基金(2006C0030M);云南省教育厅基金(06Y117B)

摘　　要：机械刺激(mechanical stimulation,MS)可提高烟草悬浮培养细胞的钙调素(calmodulin,CaM)活性,诱导烟草悬浮培养细胞耐热性的形成,外源Ca2+可加强,而Ca2+螯合剂EGTA、质膜Ca2+通道阻塞剂La3+和胞内Ca2+通道阻断剂钌红(RR),以及CaM拮抗剂氯丙嗪(CPZ)和三氟拉嗪(TFP)则削弱这种耐热性的形成。这些暗示Ca2+和CaM参与MS诱导的烟草悬浮培养细胞耐热性形成的调控。Mechanical stimulation （MS） obviously enhanced calmodulin （CAM） activity in tobacco （Nicotiana tabacum） suspension cells, induced the heat tolerance in tobacco suspension cells. The MS-induced heat toler- ance in tobacco suspension cells was strengthened with exogenous Ca2＋ treatment, but weakened by Ca2＋ chelator EGTA, plasmamembrame-channel blocker La3＋, intracellular channel blocker ruthenium red （RR） and calmodulin antagonists chlorpromazine （CPZ） and trifluoperazine （TFP）, respectively. These results suggested that Ca2＋ and CaM were involved in the regulation of MS-induced heat tolerance in tobacco suspension cells.

关 键 词：烟草悬浮培养细胞 机械刺激 耐热性 钙 钙调素 

分 类 号：Q943.1[生物学—植物学] TB53[理学—物理]