孕妇中乙型肝炎病毒前C区和核心启动子区热点变异研究  

Hot point mutations in precore/core promoter regions of HBV DNA in pregnant women

作　　者：谢新宝[1] 朱启镕[1] 王晓红[1] 

机构地区：[1]复旦大学附属儿科医院感染科,上海200032

出　　处：《肝脏》2009年第2期93-95,共3页Chinese Hepatology

基　　金：上海市卫生系统重点学科建设项目(08GWZX0102)

摘　　要：目的研究乙型肝炎病毒(HBV)携带产妇体内HBV前C区和核心启动子(CP)区热点变异情况。方法82例无症状慢性HBV携带临产孕妇采用HBV核酸扩增荧光定量检测试剂盒抽提孕妇血清HBV DNA,血清HBsAg、HBeAg采用EIA法检测,半巢式PCR法扩增产妇HBV前C区、CP区核苷酸片段,ABI3730型DNA自动荧光测序仪对PCR产物直接测序。结果82例无症状HBV携带临产孕妇中HBsAg单阳性52例,HBsAg、HBeAg双阳性30例;单阳性孕妇中1896G→A变异的检出率为21.2%(11/52),仅测到1例双阳性孕妇存在1896G→A变异,1899G→A变异仅发生在1例单阳性孕妇,且与1896G→A变异连锁出现。1762A→T/1764G→A变异总是连锁出现,且仅发生于单阳性孕妇,单阳性孕妇中双变异的检出率为17.3%(9/52)。结论HBV前C区、CP区1896G→A及1762A→T/1764G→A热点变异在无症状HBV携带单阳性孕妇中有较高的检出率,HBV前C区和核心启动子区的热点变异可能与肝损伤的严重程度无关。Objective To investigate hot point mutations in precore/ cure promoter regions of HBV DNA in pregnant women. Methods Eighty two women in labor who were asymptomatic carriers of HBV were investigated. HBV DNA quantification in serum was detected using real-time PCR. Serum markers including Ht3eAg and HBsAg of HBV were measured by EIA. Precore and core promoter （CP） regions of HBV DNA were amplified by polymerase chain reaction and then subjected to direct sequencing. Results Precore and core promoter regions of HBV DNA were amplified and sequenced successfully in 82 pregnant women （52 HBeAg negative,30 HBeAg positive）. HBV 1896G→A mutations were found in 12 pregnant women. Only one of them was HBeAg positive. The rate of 1896G→A mutations in HBeAg negative pregnant women was 21. 2% （11/52）. 1899G→A mutation was only found in a HBeAg negative pregnant woman, and was linked with 1896G→A mutation. 1762A→T mutation was always linked with 1764G→A mutation. 1762A→T/1764G→A double mutations were only found in HBeAg negative pregnant women, and the rate of it in HBeAg negative pregnant women was 17.3%（9/52）. Conclusion The incidence rate of 1896G→A and 1762A→T/ 1764G→A double mutations are relatively high in HBeAg negative pregnant women who are asymptomatic carriers of HBV, but ralatively rare in HBeAg positive women. 1899G→A mutations are relatively rare in HBeAg negative or positive women. Hot point mutations in precore/ core promoter regions of HBV DNA may not be associated with serious liver impairment.

关 键 词：乙型肝炎病毒 前C区 核心启动子区 热点变异 

分 类 号：R714.2[医药卫生—妇产科学] R512.62[医药卫生—临床医学] R450