正常孕妇与妊高症患者胎盘中Ghrelin、GHS-R mRNA的表达研究  

Expression Research of Ghrelin and its Receptor(GHS-R) mRNA in the Placenta of Normal Pregnant Women and Pregnancy-induce Hypertension Syndrome Patients

作　　者：姜丽萍[1,2,3] 刘德斌 杜晨光[1] 李海军[1] 赵鹏伟[1] 钱英红[1] 杨燕燕[1] 曹贵方[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018 [2]内蒙古医学院组织胚胎学教研室,内蒙古呼和浩特010059 [3]内蒙古自治区医院,内蒙古呼和浩特010035 [4]内蒙古自治区人民检察院技术处,内蒙古呼和浩特010011

出　　处：《安徽农业科学》2009年第12期5367-5369,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30860201)

摘　　要：[目的]通过比较正常孕妇与妊高症患者胎盘中Ghrelin及其受体GHS-R mRNA水平的变化,探讨Ghrelin及其受体与妊高症的发病是否相关。[方法]采用实时荧光定量RT-PCR技术检测了正常孕妇胎盘和妊高症患者胎盘中Ghrelin及其受体GHS-R mRNA水平相对表达量的变化。[结果]正常孕妇胎盘组织中mRNA水平与妊高症患者胎盘组织中mRNA水平无显著性差异,但Ghrelin受体GHS-R在两者之间的mRNA水平有显著性差异(P<0.05)。[结论]Ghrelin及其受体在妊高症患者和正常孕妇胎盘中表达的相对变化表明,Ghrelin受体缺乏可能成为妊高症发病的因素之一。[Objective] The aim was to discussed the correlations of Ghrelin and its receptor （GHS-R） and pregnancy-induced hypertension syndrome by comparing with nonnal pregnant women and pregnancy-induced hypertension syndrome patients in Ghrelin and its receptor （GHS-R） mRNA level changes. [Method] Ghrelin and its receptor （GHS-R） mRNA expression changes were detected in normal pregnancy women and pregnancy-induced hypertension syndrome patients by using real-time quantitative RT-PCR. [Result] The mRNA level was no significant difference between normal placenta of pregnant women and pregnancy-induced hypertension syndrome patients, however, the mRNA level of Ghrelin receptor （GHS-R） was significantly different（ P 〈 0.05）. [Conclusion] The result of expression relative changes of Ghrelin and its receptor in pregnancy-induced hypertension syndrome patients and normal placenta of pregnant women showed that the lack of Ghrelin receptor may be a risk factor of pregnancy-induced hypertension syndrome.

关 键 词：妊高症 胎盘 生长素 实时定量RT-PCR 

分 类 号：R714[医药卫生—妇产科学]