松辽黑猪脏器总RNA提取方法的改进及应用  

Improvement and Application of Total RNA Extraction Method of Songliao Black Pig Organs

作　　者：马倩[1] 张永宏[1] 秦莹[1] 朱银莉[1] 刘同欣[1] 韦有望[1] 赵志辉[1] 张嘉保[2] 高妍[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学实验动物中心,吉林长春130062

出　　处：《安徽农业科学》2009年第12期5384-5385,共2页Journal of Anhui Agricultural Sciences

基　　金：国家科技支撑计划项目(2007BAD55B03);国家高技术研究发展计划项目(2008AA10Z136)

摘　　要：[目的]改进松辽黑猪脏器总RNA的高效快速提取方法,为检测脏器组织RNA提供基础。[方法]采用液氮研磨,利用Trizol使细胞膜破裂,并通过氯仿、异丙醇、无水乙醇处理,使RNA与杂质分离,得到高质量的RNA。[结果]得到的松辽黑猪脏器总RNA浓度和纯度都比较高,并且未被降解。[结论]该方法提取的RNA质量没有DNA污染、纯度高、没有降解、完整性好,稳定可靠,并且用时少。[Objective] The aim was to improve an effective and rapid method for extracting total RNA of Songliao black pig, and provide basis for testing organs tissue RNA. [Method] Using liquid nitrogen grinding method, cell membrane was ruptured by using Trizol , through some treatments with chloroform, isopropanol and absolute alcohol, RNA was purified and high quality RNA was obtained. [Result] The concentration and purity were high of obtained total RNA of Songliao black pig, and were not degraded. [Conclusion] The quality and purity of RNA are high and not contaminated by DNA by using this method, which has better integrity and stability, and could save time.

关 键 词：松辽黑猪 脏器 总RNA 提取 

分 类 号：S828[农业科学—畜牧学]