绵羊肌肉IGF-IR基因表达的发育性变化研究  

Study on Developmental Change of Muscle IGF-IR Gene Expression in Sheep

作　　者：黄治国[1,2] 谢庄[2] 

机构地区：[1]四川理工学院酿酒生物技术及应用四川省重点实验室,四川自贡643000 [2]南京农业大学动物科技学院,江苏南京210095

出　　处：《安徽农业科学》2009年第12期5406-5408,共3页Journal of Anhui Agricultural Sciences

基　　金：新疆建设兵团博士基金(2003-02)

摘　　要：[目的]为绵羊的生长发育研究提供基础资料。[方法]以2、30、60、90、120日龄雄性哈萨克羊和2、30-60、90日龄新疆细毛羊为试验动物，对其肌肉IGF-IR基因进行PCR扩增，并对扩增产物进行电泳检测和序列分析。[结果]绵羊肌肉IGF-IR基因PCR扩增产物在琼脂糖凝胶上出现了287和379bp2条带，该基因与牛IGF-IR基因序列的同源性为98．59％；在整个研究过程中，哈萨克羊1GF-IRmRNA表达量较低、较稳定，仅在30日龄时略有上升；新疆细毛羊IGF-IRmRNA表达量在2日龄时较高，30日龄时略有下降，60日龄时达到最高峰，90日龄时降到最低水平，且各日龄表达量差异显著；哈萨克羊mRNA表达量在2～60日龄时显著低于新疆细毛羊，而90日龄时显著高于新疆细毛羊。[结论]绵羊肌肉IGF-IR基因表达存在较大的品种间差异。[Objective] The study was to provide the basic data for studying growth and development of sheep. [Method] With kazak male sheep of 2, 30, 60, 90, 120 day-old and Xinjiang fine wool sheep of 2, 30, 60, 90 day-old as the tested animal, the IGF-IR gene in their muscle was amplified with PCR, and the amplified products was detected by electrophoresis and its sequence was analyzed. [Result] The PCR amplified products of IGF-IR gene appeared 2 strips with 287 and 379 by in agarose gel. The homology of IGF-IR gene in sheep and cow was 98.59% . During the whole research process, the expression level of IGF-IR mRNA in Kazakh sheep was lower and stable and slightly increased at 30 day-old. The expression level of IGF-IR mRNA in Xinjiang fine wool sheep was higher at 2 day-old, and slightly decreased at 30 day-old and reached the highest peak at 60 day-old, and decreased to the lowest level at 90 day-old, and the expression level at different. day-old was significantly different . The expression level of mRNA in Kazakh at 2 - 60 dayold was obviously lower than that in Xinjiang fine wool sheep, and was significantly higher than that in Xinjiang fine wool sheep at 90 day-old. [Conclusion] The IGF-IR gene expression in sheep muscle had big difference between varieties.

关 键 词：绵羊 肌肉 IGF-IR基因 表达 

分 类 号：S826[农业科学—畜牧学]